Abstract
The impact of the use of herbicides in agriculture can be minimized by compliance with good management practices that reduce the amount used and their release into the environment. Simple tests that provide real time on-site information about these chemicals are a major aid for these programs. In this work, we show that phage anti-immunocomplex assay (PHAIA), a method that uses phage-borne peptides to detect the formation of antibody-analyte immunocomplexes, is an advantageous technology to produce such field tests. A monoclonal antibody to the herbicide clomazone was raised and used in the development of conventional competitive and noncompetitive PHAIA immunoassays. The sensitivity attained with the PHAIA format was over 10 times higher than that of the competitive format. The cross-reactivity of the two methods was also compared using structurally related compounds, and we observed that the two-site binding of PHAIA "double-checks" the recognition of the analyte, thereby increasing the assay specificity. The positive readout of the noncompetitive PHAIA method allowed adaptation of the assay into a rapid and simple format where as little as 0.4 ng/mL clomazone (more than 10-fold lower than the proposed standard) in water samples from a rice field could be easily detected by simple visual inspection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8838-8843 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 82 |
| Issue number | 21 |
| DOIs | |
| State | Published - Nov 1 2010 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Phage anti-immunocomplex assay for clomazone : Two-site recognition increasing assay specificity and facilitating adaptation into an on-site format. / Rossotti, M. A.; Carlomagno, M.; González-Techera, A.; Hammock, B. D.; Last, Jerold A; González-Sapienza, G.
In: Analytical Chemistry, Vol. 82, No. 21, 01.11.2010, p. 8838-8843.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phage anti-immunocomplex assay for clomazone
T2 - Two-site recognition increasing assay specificity and facilitating adaptation into an on-site format
AU - Rossotti, M. A.
AU - Carlomagno, M.
AU - González-Techera, A.
AU - Hammock, B. D.
AU - Last, Jerold A
AU - González-Sapienza, G.
PY - 2010/11/1
Y1 - 2010/11/1
N2 - The impact of the use of herbicides in agriculture can be minimized by compliance with good management practices that reduce the amount used and their release into the environment. Simple tests that provide real time on-site information about these chemicals are a major aid for these programs. In this work, we show that phage anti-immunocomplex assay (PHAIA), a method that uses phage-borne peptides to detect the formation of antibody-analyte immunocomplexes, is an advantageous technology to produce such field tests. A monoclonal antibody to the herbicide clomazone was raised and used in the development of conventional competitive and noncompetitive PHAIA immunoassays. The sensitivity attained with the PHAIA format was over 10 times higher than that of the competitive format. The cross-reactivity of the two methods was also compared using structurally related compounds, and we observed that the two-site binding of PHAIA "double-checks" the recognition of the analyte, thereby increasing the assay specificity. The positive readout of the noncompetitive PHAIA method allowed adaptation of the assay into a rapid and simple format where as little as 0.4 ng/mL clomazone (more than 10-fold lower than the proposed standard) in water samples from a rice field could be easily detected by simple visual inspection.
AB - The impact of the use of herbicides in agriculture can be minimized by compliance with good management practices that reduce the amount used and their release into the environment. Simple tests that provide real time on-site information about these chemicals are a major aid for these programs. In this work, we show that phage anti-immunocomplex assay (PHAIA), a method that uses phage-borne peptides to detect the formation of antibody-analyte immunocomplexes, is an advantageous technology to produce such field tests. A monoclonal antibody to the herbicide clomazone was raised and used in the development of conventional competitive and noncompetitive PHAIA immunoassays. The sensitivity attained with the PHAIA format was over 10 times higher than that of the competitive format. The cross-reactivity of the two methods was also compared using structurally related compounds, and we observed that the two-site binding of PHAIA "double-checks" the recognition of the analyte, thereby increasing the assay specificity. The positive readout of the noncompetitive PHAIA method allowed adaptation of the assay into a rapid and simple format where as little as 0.4 ng/mL clomazone (more than 10-fold lower than the proposed standard) in water samples from a rice field could be easily detected by simple visual inspection.
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UR - http://www.scopus.com/inward/citedby.url?scp=78049496942&partnerID=8YFLogxK
U2 - 10.1021/ac101476f
DO - 10.1021/ac101476f
M3 - Article
C2 - 20886819
AN - SCOPUS:78049496942
VL - 82
SP - 8838
EP - 8843
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 21
ER -