Abstract
Objective: To determine whether levels of PH-20, a hyaluronidase similar to that found in human sperm, are elevated in laryngeal cancer tissue. Design: In this case-control study, reverse transcription polymerase chain reaction was used to measure levels of PH-20 messenger RNA in tissue taken from laryngectomy specimens. Setting: A university medical center. Patients: We compared tissue samples taken from 11 patients with laryngeal cancer, and from 2 metastatic lymph nodes, with samples of normal, healthy laryngeal tissue and prostate cancer tissue (positive control). Main Outcome Measure: PH-20 complementary DNA expression as quantified by densitometric analysis. Results: Expression of PH-20 was significantly higher in nonirradiated laryngeal cancer specimens than in normal laryngeal tissue (P<.01). Metastatic lymph nodes also had higher levels of PH-20 expression than did primary laryngeal cancer tissue (P = .11) and normal laryngeal tissue (P<.01). Irradiated laryngeal cancer specimens had PH-20 levels comparable to normal. Conclusions: We report the first data on PH-20 expression in laryngeal cancer tissue. PH-20 expression is significantly elevated in primary laryngeal cancer tissue and seems to be even higher in metastatic lesions compared with normal laryngeal tissue. PH-20 may be a useful tumor marker and prognostic tool for laryngeal cancer.
Original language | English (US) |
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Pages (from-to) | 402-404 |
Number of pages | 3 |
Journal | Archives of Otolaryngology - Head and Neck Surgery |
Volume | 126 |
Issue number | 3 |
State | Published - Mar 2000 |
Externally published | Yes |
ASJC Scopus subject areas
- Otorhinolaryngology