pet 18

A chromosomal gene required for cell growth and for the maintenance of mitochondrial DNA and the killer plasmid of yeast

Michael J Leibowitz, Reed B. Wickner

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mutations in the pet18 gene of Saccharomyces cerevisiae (formerly denoted pets) confer three phenotypes on mutant strains: (i) inability to respire (petite), (ii) inability to maintain the double-stranded RNA killer plasmid (sensitive), and (iii) temperature sensitivity for growth. We find that pet18 mutants lack mitochondrial DNA. However, despite their inability to maintain the killer RNA plasmid and mitochondrial DNA, pet18 mutants still can carry the other yeast plasmids, [URE3-1], [PSI], and 2-micron DNA. The temperature sensitivity of the pet18 mutants is not expressed as a selective defect in total DNA, RNA, or protein synthesis.

Original languageEnglish (US)
Pages (from-to)115-121
Number of pages7
JournalMGG Molecular & General Genetics
Volume165
Issue number2
DOIs
StatePublished - Jan 1978
Externally publishedYes

Fingerprint

Pets
Mitochondrial DNA
Plasmids
Yeasts
Maintenance
Growth
RNA
Genes
Temperature
Double-Stranded RNA
DNA
Saccharomyces cerevisiae
Phenotype
Mutation
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

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AB - Mutations in the pet18 gene of Saccharomyces cerevisiae (formerly denoted pets) confer three phenotypes on mutant strains: (i) inability to respire (petite), (ii) inability to maintain the double-stranded RNA killer plasmid (sensitive), and (iii) temperature sensitivity for growth. We find that pet18 mutants lack mitochondrial DNA. However, despite their inability to maintain the killer RNA plasmid and mitochondrial DNA, pet18 mutants still can carry the other yeast plasmids, [URE3-1], [PSI], and 2-micron DNA. The temperature sensitivity of the pet18 mutants is not expressed as a selective defect in total DNA, RNA, or protein synthesis.

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