Pervasive and essential roles of the top3-rmi1 decatenase orchestrate recombination and facilitate chromosome segregation in meiosis

Shangming Tang, Michelle Ka Yan Wu, Ruoxi Zhang, Neil Hunter

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The Bloom's helicase ortholog, Sgs1, plays central roles to coordinate the formation and resolution of joint molecule intermediates (JMs) during meiotic recombination in budding yeast. Sgs1 can associate with type-I topoisomerase Top3 and its accessory factor Rmi1 to form a conserved complex best known for its unique ability to decatenate double-Holliday junctions. Contrary to expectations, we show that the strand-passage activity of Top3-Rmi1 is required for all known functions of Sgs1 in meiotic recombination, including channeling JMs into physiological crossover and noncrossover pathways, and suppression of non-allelic recombination. We infer that Sgs1 always functions in the context of the Sgs1-Top3-Rmi1 complex to regulate meiotic recombination. In addition, we reveal a distinct late role for Top3-Rmi1 in resolving recombination-dependent chromosome entanglements to allow segregation at anaphase. Surprisingly, Sgs1 does not share this essential role of Top3-Rmi1. These data reveal an essential and pervasive role for the Top3-Rmi1 decatenase during meiosis.

Original languageEnglish (US)
Pages (from-to)607-621
Number of pages15
JournalMolecular Cell
Volume57
Issue number4
DOIs
StatePublished - Feb 19 2015

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Chromosome Segregation
Meiosis
Genetic Recombination
Cruciform DNA
Type I DNA Topoisomerase
Anaphase
Saccharomycetales
Chromosomes
Joints

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Pervasive and essential roles of the top3-rmi1 decatenase orchestrate recombination and facilitate chromosome segregation in meiosis. / Tang, Shangming; Wu, Michelle Ka Yan; Zhang, Ruoxi; Hunter, Neil.

In: Molecular Cell, Vol. 57, No. 4, 19.02.2015, p. 607-621.

Research output: Contribution to journalArticle

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