Persistent Progenitors at the Retinal Margin of ptc+/- Mice

Ala Moshiri, Thomas A. Reh

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

The hedgehog signaling pathway is a key regulator of neural development, affecting both proliferation and differentiation of neural progenitors. Sonic hedgehog (Shh) is a mitogenic factor for retinal progenitors in vitro. To determine whether this signaling system is important in vivo for regulating retinal progenitor proliferation, we analyzed mice with a single functional allele of the Shh receptor patched (ptc). We found that ptc+/- mice had increased numbers of neural progenitors at every stage of retinal development that we examined. In addition, these mice had persistent progenitors at the retinal margin for up to 3 months of age, reminiscent of the ciliary marginal zone of lower vertebrates. To test whether the progenitors at the retinal margin of ptc+/- mice could be induced to regenerate retinal neurons in response to damage, we bred ptc+/- mice onto a retinal degeneration background (pro23his rhodopsin transgenic) and labeled newly generated cells with combined immunohistochemistry for bromodeoxyuridine and retinal neuron and photoreceptor-specific markers. We found newly generated neurons and photoreceptors at the retinal margin in ptc+/-;pro23his mice. We propose that the Shh pathway may act as a regulator of both prenatal and postnatal retinal growth.

Original languageEnglish (US)
Pages (from-to)229-237
Number of pages9
JournalJournal of Neuroscience
Volume24
Issue number1
DOIs
StatePublished - Jan 7 2004
Externally publishedYes

Keywords

  • Development
  • Neurogenesis
  • Progenitor
  • Regeneration
  • Retina
  • Stem cell

ASJC Scopus subject areas

  • Neuroscience(all)

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