Persistent infection of rabbits with HTLV-1: Patterns of anti-viral antibody reactivity and detection of virus by gene amplification

G. L. Cockerell, Michael Dale Lairmore, B. De, J. Rovnak, T. M. Hartley, I. Miyoshi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Two groups of rabbits were inoculated on the day of birth or at 4 weeks of age with a human T-cell leukemia virus type-I (HTLV-I)-infected and transformed rabbit cell line (Ra-I). Rabbits seroconverted to HTLV-I, as determined by indirect immunofluorescence, by 3 weeks after inoculation and remained persistently seropositive during a 22-month period of observation. Seroconversion did not occur in saline-inoculated controls. Using Western immunoblotting and radio-immunoprecipitation, persistent seroconversion occurred against viral antigens p24, p55 and gp68, while reactivity to p19 was variable between rabbits. Using the polymerase chain reaction (PCR) with HTLV-I gag and pol primer pairs, HTLV-I sequences were demonstrable in peripheral blood mononuclear cells and other tissues collected at 70 and 90 weeks after inoculation. DNA extracts from normal rabbit tissue remained negative under the same conditions. No qualitative or quantitative changes in leukocytes or erythrocytes were detected in the infected rabbits and no clinical signs could be directly attributed to infection with HTLV-I.

Original languageEnglish (US)
Pages (from-to)127-130
Number of pages4
JournalInternational Journal of Cancer
Volume45
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

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Viral Antibodies
Human T-lymphotropic virus 1
Gene Amplification
Anti-Idiotypic Antibodies
Rabbits
Viruses
Infection
HTLV-I Infections
Transformed Cell Line
Viral Antigens
Indirect Fluorescent Antibody Technique
Radio
Immunoprecipitation
Blood Cells
Leukocytes
Erythrocytes
Western Blotting
Observation
Parturition
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Persistent infection of rabbits with HTLV-1 : Patterns of anti-viral antibody reactivity and detection of virus by gene amplification. / Cockerell, G. L.; Lairmore, Michael Dale; De, B.; Rovnak, J.; Hartley, T. M.; Miyoshi, I.

In: International Journal of Cancer, Vol. 45, No. 1, 1990, p. 127-130.

Research output: Contribution to journalArticle

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AU - De, B.

AU - Rovnak, J.

AU - Hartley, T. M.

AU - Miyoshi, I.

PY - 1990

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