Persistence of cellulitis-associated Escherichia coli DNA fingerprints in successive broiler chicken flocks

Randall S. Singer, Joan S. Jeffrey, Tim Carpenter, Cara L. Cooke, Edward R Atwill, Wesley O. Johnson, Dwight C. Hirsh

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Avian cellulitis in broiler chickens is primarily caused by Escherichia coli. Previous research found that the E. coli isolates of cellulitis origin were unique to each ranch, suggesting that these E. coli were endemic within the ranch environment. To test the hypothesis that the E. coli associated with cellulitis are endemic in the litter of the broiler house, we designed a study to determine whether E. coli DNA fingerprints associated with cellulitis persist over successive flocks that are grown in the same house. In addition, we assessed the impact of different cleaning and disinfection strategies on this persistence. Two broiler houses were followed on each of five farms over 3-4 flocks. A total of 353 E. coli isolates from cellulitis lesions were analyzed in this study, and 314 of these isolates (89%) were DNA fingerprinted by PFGE. In each ranch, there were several DNA fingerprint patterns that were present over successive flocks, regardless of the cleaning and disinfection strategy utilized. Isolates persisted as long as 191 days, implying that these E. coli are capable of persisting in the broiler house environment for long periods of time. In addition, these E. coli isolates were associated with cellulitis lesions in successive flocks. Thus, the isolates of E. coli that are associated with cellulitis in broiler chickens appear to be endemic in the litter environment of the broiler house. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)59-71
Number of pages13
JournalVeterinary Microbiology
Volume75
Issue number1
DOIs
StatePublished - Jul 3 2000

Fingerprint

cellulitis
Cellulitis
DNA Fingerprinting
DNA fingerprinting
Chickens
flocks
broiler chickens
Escherichia coli
ranching
Disinfection
disinfection
cleaning
farms

Keywords

  • Cellulitis
  • Chicken-Bacteria
  • Escherichia coli
  • Molecular epidemiology
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Persistence of cellulitis-associated Escherichia coli DNA fingerprints in successive broiler chicken flocks. / Singer, Randall S.; Jeffrey, Joan S.; Carpenter, Tim; Cooke, Cara L.; Atwill, Edward R; Johnson, Wesley O.; Hirsh, Dwight C.

In: Veterinary Microbiology, Vol. 75, No. 1, 03.07.2000, p. 59-71.

Research output: Contribution to journalArticle

Singer, Randall S. ; Jeffrey, Joan S. ; Carpenter, Tim ; Cooke, Cara L. ; Atwill, Edward R ; Johnson, Wesley O. ; Hirsh, Dwight C. / Persistence of cellulitis-associated Escherichia coli DNA fingerprints in successive broiler chicken flocks. In: Veterinary Microbiology. 2000 ; Vol. 75, No. 1. pp. 59-71.
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