Persistence of borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection

Monica E. Embers, Stephen W Barthold, Juan T. Borda, Lisa Bowers, Lara Doyle, Emir Hodzic, Mary B. Jacobs, Nicole R. Hasenkampf, Dale S. Martin, Sukanya Narasimhan, Kathrine M. Phillippi-Falkenstein, Jeanette E. Purcell, Marion S. Ratterree, Mario T. Philipp

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The persistence of symptoms in Lyme disease patients following antibiotic therapy, and their causes, continue to be a matter of intense controversy. The studies presented here explore antibiotic efficacy using nonhuman primates. Rhesus macaques were infected with B. burgdorferi and a portion received aggressive antibiotic therapy 4-6 months later. Multiple methods were utilized for detection of residual organisms, including the feeding of lab-reared ticks on monkeys (xenodiagnosis), culture, immunofluorescence and PCR. Antibody responses to the B. burgdorferi-specific C6 diagnostic peptide were measured longitudinally and declined in all treated animals. B. burgdorferi antigen, DNA and RNA were detected in the tissues of treated animals. Finally, small numbers of intact spirochetes were recovered by xenodiagnosis from treated monkeys. These results demonstrate that B. burgdorferi can withstand antibiotic treatment, administered post-dissemination, in a primate host. Though B. burgdorferi is not known to possess resistance mechanisms and is susceptible to the standard antibiotics (doxycycline, ceftriaxone) in vitro, it appears to become tolerant post-dissemination in the primate host. This finding raises important questions about the pathogenicity of antibiotic-tolerant persisters and whether or not they can contribute to symptoms post-treatment.

Original languageEnglish (US)
Article numbere29914
JournalPLoS One
Volume7
Issue number1
DOIs
StatePublished - Jan 11 2012

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Borrelia burgdorferi
Macaca mulatta
antibiotics
Anti-Bacterial Agents
xenodiagnosis
Xenodiagnosis
Infection
infection
Primates
signs and symptoms (animals and humans)
Haplorhini
monkeys
Animals
Therapeutics
ceftriaxone
Spirochaetales
therapeutics
doxycycline
Lyme disease
Lyme Disease

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Persistence of borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection. / Embers, Monica E.; Barthold, Stephen W; Borda, Juan T.; Bowers, Lisa; Doyle, Lara; Hodzic, Emir; Jacobs, Mary B.; Hasenkampf, Nicole R.; Martin, Dale S.; Narasimhan, Sukanya; Phillippi-Falkenstein, Kathrine M.; Purcell, Jeanette E.; Ratterree, Marion S.; Philipp, Mario T.

In: PLoS One, Vol. 7, No. 1, e29914, 11.01.2012.

Research output: Contribution to journalArticle

Embers, ME, Barthold, SW, Borda, JT, Bowers, L, Doyle, L, Hodzic, E, Jacobs, MB, Hasenkampf, NR, Martin, DS, Narasimhan, S, Phillippi-Falkenstein, KM, Purcell, JE, Ratterree, MS & Philipp, MT 2012, 'Persistence of borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection', PLoS One, vol. 7, no. 1, e29914. https://doi.org/10.1371/journal.pone.0029914
Embers, Monica E. ; Barthold, Stephen W ; Borda, Juan T. ; Bowers, Lisa ; Doyle, Lara ; Hodzic, Emir ; Jacobs, Mary B. ; Hasenkampf, Nicole R. ; Martin, Dale S. ; Narasimhan, Sukanya ; Phillippi-Falkenstein, Kathrine M. ; Purcell, Jeanette E. ; Ratterree, Marion S. ; Philipp, Mario T. / Persistence of borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection. In: PLoS One. 2012 ; Vol. 7, No. 1.
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