Persistence of Borrelia burgdorferi following antibiotic treatment in mice

Emir Hodzic, Sunlian Feng, Kevin Holden, Kimberly J. Freet, Stephen W Barthold

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The effectiveness of antibiotic treatment was examined in a mouse model of Lyme borreliosis. Mice were treated with ceftriaxone or saline solution for 1 month, commencing during the early (3 weeks) or chronic (4 months) stages of infection with Borrelia burgdorferi. Tissues from mice were tested for infection by culture, PCR, xenodiagnosis, and transplantation of allografts at 1 and 3 months after completion of treatment. In addition, tissues were examined for the presence of spirochetes by immunohistochemistry. In contrast to saline solution-treated mice, mice treated with antibiotic were consistently culture negative, but tissues from some of the mice remained PCR positive, and spirochetes could be visualized in collagen-rich tissues. Furthermore, when some of the antibiotic-treated mice were fed on by Ixodes scapularis ticks (xenodiagnosis), spirochetes were acquired by the ticks, as determined based upon PCR results, and ticks from those cohorts transmitted spirochetes to naïve SCID mice, which became PCR positive but culture negative. Results indicated that following antibiotic treatment, mice remained infected with nondividing but infectious spirochetes, particularly when antibiotic treatment was commenced during the chronic stage of infection.

Original languageEnglish (US)
Pages (from-to)1728-1736
Number of pages9
JournalAntimicrobial Agents and Chemotherapy
Volume52
Issue number5
DOIs
StatePublished - May 2008

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Borrelia burgdorferi
Spirochaetales
Anti-Bacterial Agents
Ticks
Xenodiagnosis
Polymerase Chain Reaction
Therapeutics
Sodium Chloride
Infection
Ixodes
SCID Mice
Lyme Disease
Ceftriaxone
Allografts
Collagen
Transplantation
Immunohistochemistry

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Persistence of Borrelia burgdorferi following antibiotic treatment in mice. / Hodzic, Emir; Feng, Sunlian; Holden, Kevin; Freet, Kimberly J.; Barthold, Stephen W.

In: Antimicrobial Agents and Chemotherapy, Vol. 52, No. 5, 05.2008, p. 1728-1736.

Research output: Contribution to journalArticle

Hodzic, Emir ; Feng, Sunlian ; Holden, Kevin ; Freet, Kimberly J. ; Barthold, Stephen W. / Persistence of Borrelia burgdorferi following antibiotic treatment in mice. In: Antimicrobial Agents and Chemotherapy. 2008 ; Vol. 52, No. 5. pp. 1728-1736.
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