Permeability coefficients of the capillary wall to low molecular weight hydrophilic solutes measured in single perfused capillaries of frog mesentery

F. E. Curry

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Individual capillaries of frog mesentery were perfused using a micropipet and superfused with frog Ringer containing the test solutes. Following occlusion of the capillary, the initial osmotic flows per unit area of capillary, (ΔJv/S)0, were measured using the methods of Curry et al. (J. Physiol. 261, 319-336). When the perfusion rate, Q, was high, (ΔJv/S)0 was independent of Q; at lower perfusion rates, (ΔJv/S)0 decreased as Q decreased. Permeability coefficients, P. were calculated from the fractional reduction of the osmotic flows in capillaries where both perfusion rate and surface area were measured directly. Capillary filtration coefficients (Lp) and osmotic reflection coefficients (σ) were also determined. Calculations based on measured σs showed that the effect of solute sieving on the estimates of P was small. Three experiments to measure directly the concentration of potassium ion in the fluid on both sides on the capillary wall indicate the diffusion resistance outside the capillary wall was negligible. At least two solutes were tested on each capillary. At 14-18° the mean PNaCl was 4.40 ± 0.67 (range 1.25-10.8) × 10-4 cm sec-1 (15 capillaries). The mean Psuctuse was 1.43 ± 0.36 (range 0.32-4.8) × 10-4 (13). Urea, glucose, and raffinose were test solutes in smaller samples. What factors determine the number of pathways for water and hydrophilic solutes (of approximately constant equivalent pore size) across the walls of continuous capillaries?

Original languageEnglish (US)
Pages (from-to)290-308
Number of pages19
JournalMicrovascular Research
Volume17
Issue number3
DOIs
StatePublished - 1979

Fingerprint

Raffinose
Mesentery
Hydraulic conductivity
Capillary Permeability
Anura
Pore size
Urea
Potassium
Molecular Weight
Molecular weight
Ions
Glucose
Fluids
Water
Experiments
Perfusion
Capillary Resistance
Permeability

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Cardiology and Cardiovascular Medicine

Cite this

@article{0e4b4aafe7ee48929464bd885d29db9f,
title = "Permeability coefficients of the capillary wall to low molecular weight hydrophilic solutes measured in single perfused capillaries of frog mesentery",
abstract = "Individual capillaries of frog mesentery were perfused using a micropipet and superfused with frog Ringer containing the test solutes. Following occlusion of the capillary, the initial osmotic flows per unit area of capillary, (ΔJv/S)0, were measured using the methods of Curry et al. (J. Physiol. 261, 319-336). When the perfusion rate, Q, was high, (ΔJv/S)0 was independent of Q; at lower perfusion rates, (ΔJv/S)0 decreased as Q decreased. Permeability coefficients, P. were calculated from the fractional reduction of the osmotic flows in capillaries where both perfusion rate and surface area were measured directly. Capillary filtration coefficients (Lp) and osmotic reflection coefficients (σ) were also determined. Calculations based on measured σs showed that the effect of solute sieving on the estimates of P was small. Three experiments to measure directly the concentration of potassium ion in the fluid on both sides on the capillary wall indicate the diffusion resistance outside the capillary wall was negligible. At least two solutes were tested on each capillary. At 14-18° the mean PNaCl was 4.40 ± 0.67 (range 1.25-10.8) × 10-4 cm sec-1 (15 capillaries). The mean Psuctuse was 1.43 ± 0.36 (range 0.32-4.8) × 10-4 (13). Urea, glucose, and raffinose were test solutes in smaller samples. What factors determine the number of pathways for water and hydrophilic solutes (of approximately constant equivalent pore size) across the walls of continuous capillaries?",
author = "Curry, {F. E.}",
year = "1979",
doi = "10.1016/S0026-2862(79)80005-9",
language = "English (US)",
volume = "17",
pages = "290--308",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Permeability coefficients of the capillary wall to low molecular weight hydrophilic solutes measured in single perfused capillaries of frog mesentery

AU - Curry, F. E.

PY - 1979

Y1 - 1979

N2 - Individual capillaries of frog mesentery were perfused using a micropipet and superfused with frog Ringer containing the test solutes. Following occlusion of the capillary, the initial osmotic flows per unit area of capillary, (ΔJv/S)0, were measured using the methods of Curry et al. (J. Physiol. 261, 319-336). When the perfusion rate, Q, was high, (ΔJv/S)0 was independent of Q; at lower perfusion rates, (ΔJv/S)0 decreased as Q decreased. Permeability coefficients, P. were calculated from the fractional reduction of the osmotic flows in capillaries where both perfusion rate and surface area were measured directly. Capillary filtration coefficients (Lp) and osmotic reflection coefficients (σ) were also determined. Calculations based on measured σs showed that the effect of solute sieving on the estimates of P was small. Three experiments to measure directly the concentration of potassium ion in the fluid on both sides on the capillary wall indicate the diffusion resistance outside the capillary wall was negligible. At least two solutes were tested on each capillary. At 14-18° the mean PNaCl was 4.40 ± 0.67 (range 1.25-10.8) × 10-4 cm sec-1 (15 capillaries). The mean Psuctuse was 1.43 ± 0.36 (range 0.32-4.8) × 10-4 (13). Urea, glucose, and raffinose were test solutes in smaller samples. What factors determine the number of pathways for water and hydrophilic solutes (of approximately constant equivalent pore size) across the walls of continuous capillaries?

AB - Individual capillaries of frog mesentery were perfused using a micropipet and superfused with frog Ringer containing the test solutes. Following occlusion of the capillary, the initial osmotic flows per unit area of capillary, (ΔJv/S)0, were measured using the methods of Curry et al. (J. Physiol. 261, 319-336). When the perfusion rate, Q, was high, (ΔJv/S)0 was independent of Q; at lower perfusion rates, (ΔJv/S)0 decreased as Q decreased. Permeability coefficients, P. were calculated from the fractional reduction of the osmotic flows in capillaries where both perfusion rate and surface area were measured directly. Capillary filtration coefficients (Lp) and osmotic reflection coefficients (σ) were also determined. Calculations based on measured σs showed that the effect of solute sieving on the estimates of P was small. Three experiments to measure directly the concentration of potassium ion in the fluid on both sides on the capillary wall indicate the diffusion resistance outside the capillary wall was negligible. At least two solutes were tested on each capillary. At 14-18° the mean PNaCl was 4.40 ± 0.67 (range 1.25-10.8) × 10-4 cm sec-1 (15 capillaries). The mean Psuctuse was 1.43 ± 0.36 (range 0.32-4.8) × 10-4 (13). Urea, glucose, and raffinose were test solutes in smaller samples. What factors determine the number of pathways for water and hydrophilic solutes (of approximately constant equivalent pore size) across the walls of continuous capillaries?

UR - http://www.scopus.com/inward/record.url?scp=0018747469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018747469&partnerID=8YFLogxK

U2 - 10.1016/S0026-2862(79)80005-9

DO - 10.1016/S0026-2862(79)80005-9

M3 - Article

C2 - 459941

AN - SCOPUS:0018747469

VL - 17

SP - 290

EP - 308

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

IS - 3

ER -