Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes

N. Songsasen, M. S. Ratterree, C. A. Vandevoort, D. E. Pegg, S. P. Leibo

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Permeability characteristics and sensitivity to osmotic shock are principal parameters that are important to derive procedures for the successful cryopreservation of mammalian oocytes. Methods and results: The osmotically inactive volume of rhesus monkey oocytes was determined by measuring their volumes in the presence of hypertonic solutions of sucrose from 0.2 to 1.5 mol/l, compared with their volume in isotonic TALP-HEPES solution. Boyle-van't Hoff plots at infinite osmolality indicated that the non-osmotic volumes of immature and mature oocytes were 20 and 17% respectively. Osmotic responses of oocytes exposed to 1.0 mol/l solutions of glycerol, dimethylsulphoxide (DMSO) and ethylene glycol (EG) were determined. Rhesus monkey oocytes appeared to be less permeable to glycerol than to DMSO or to EG. Sensitivity of oocytes to osmotic shock was determined by exposing them to various solutions of EG (0.1 to 5.0 mol/l) and then abruptly diluting them into isotonic medium. Morphological survival, as measured by membrane integrity, of oocytes diluted out of EG depended significantly on the concentration of EG (P < 0.01). Conclusion: Determination of permeability characteristics and sensitivity to osmotic shock of rhesus oocytes will aid in the derivation of procedures for their cryopreservation.

Original languageEnglish (US)
Pages (from-to)1875-1884
Number of pages10
JournalHuman Reproduction
Volume17
Issue number7
StatePublished - 2002

Fingerprint

Macaca mulatta
Oocytes
Permeability
Ethylene Glycol
Osmotic Pressure
Cryopreservation
Dimethyl Sulfoxide
Glycerol
HEPES
Hypertonic Solutions
Osmolar Concentration
Sucrose
Membranes

Keywords

  • Oocytes
  • Osmotic shock sensitivity
  • Permeability characteristics
  • Rhesus monkey

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Songsasen, N., Ratterree, M. S., Vandevoort, C. A., Pegg, D. E., & Leibo, S. P. (2002). Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes. Human Reproduction, 17(7), 1875-1884.

Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes. / Songsasen, N.; Ratterree, M. S.; Vandevoort, C. A.; Pegg, D. E.; Leibo, S. P.

In: Human Reproduction, Vol. 17, No. 7, 2002, p. 1875-1884.

Research output: Contribution to journalArticle

Songsasen, N, Ratterree, MS, Vandevoort, CA, Pegg, DE & Leibo, SP 2002, 'Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes', Human Reproduction, vol. 17, no. 7, pp. 1875-1884.
Songsasen N, Ratterree MS, Vandevoort CA, Pegg DE, Leibo SP. Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes. Human Reproduction. 2002;17(7):1875-1884.
Songsasen, N. ; Ratterree, M. S. ; Vandevoort, C. A. ; Pegg, D. E. ; Leibo, S. P. / Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes. In: Human Reproduction. 2002 ; Vol. 17, No. 7. pp. 1875-1884.
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