Perinatal physiology in cloned and normal calves: Hematologic and biochemical profiles

Cynthia A. Batchelder, Marcelo Bertolini, Jeffrey B. Mason, Alice L. Moyer, Kara A. Hoffert, Stoyan G. Petkov, Thomas R. Famula, John A Angelos, Lisle W. George, Gary B. Anderson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although a majority of clones are born normal and apparently healthy, mortality rates of nearly 30% are described in many reports. Such losses are a major limitation of cloning technology and represent substantial economic investment as well as justifiable animal health and welfare concerns. Prospective, controlled studies are needed to understand fully the causes of neonatal mortality in clones and to develop preventive and therapeutic strategies to minimize losses. We report here the findings of studies on the hematologic and biochemical profiles of cloned and control calves in the immediate 48-h postpartum period. Cloned calves were similar to control calves for a majority of parameters studied including blood gases, concentrations of plasma proteins, minerals and electrolytes, and white blood cell, neutrophil, lymphocyte, and platelet counts. The most notable differences between clones and controls in this study were reduced red- and white-blood cell counts in clones at birth and 1 h of age. As a group, plasma electrolyte concentrations were more variable in clones, and the variability tended to be shifted either higher (sodium, chloride) or lower (potassium, bicarbonate) than in controls. Previously, we noted differences in carbohydrate parameters, the length of time required for clones to make the neonatal adaptation to life ex utero, and morphology of the cloned placenta. Taken together, our findings suggest that cloned calves experience greater difficulty adjusting to life ex utero and that further research is warranted to determine the nature of the relationship between the physiological differences noted here in clones at birth and concomitant abnormal placental morphology.

Original languageEnglish (US)
Pages (from-to)83-96
Number of pages14
JournalCloning and Stem Cells
Volume9
Issue number1
DOIs
StatePublished - 2007

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Clone Cells
Leukocyte Count
Electrolytes
Parturition
Animal Welfare
Erythrocyte Count
Lymphocyte Count
Infant Mortality
Platelet Count
Sodium Chloride
Postpartum Period
Placenta
Minerals
Organism Cloning
Blood Proteins
Neutrophils
Gases
Economics
Carbohydrates
Prospective Studies

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Applied Microbiology and Biotechnology

Cite this

Batchelder, C. A., Bertolini, M., Mason, J. B., Moyer, A. L., Hoffert, K. A., Petkov, S. G., ... Anderson, G. B. (2007). Perinatal physiology in cloned and normal calves: Hematologic and biochemical profiles. Cloning and Stem Cells, 9(1), 83-96. https://doi.org/10.1089/clo.2006.0038

Perinatal physiology in cloned and normal calves : Hematologic and biochemical profiles. / Batchelder, Cynthia A.; Bertolini, Marcelo; Mason, Jeffrey B.; Moyer, Alice L.; Hoffert, Kara A.; Petkov, Stoyan G.; Famula, Thomas R.; Angelos, John A; George, Lisle W.; Anderson, Gary B.

In: Cloning and Stem Cells, Vol. 9, No. 1, 2007, p. 83-96.

Research output: Contribution to journalArticle

Batchelder, CA, Bertolini, M, Mason, JB, Moyer, AL, Hoffert, KA, Petkov, SG, Famula, TR, Angelos, JA, George, LW & Anderson, GB 2007, 'Perinatal physiology in cloned and normal calves: Hematologic and biochemical profiles', Cloning and Stem Cells, vol. 9, no. 1, pp. 83-96. https://doi.org/10.1089/clo.2006.0038
Batchelder CA, Bertolini M, Mason JB, Moyer AL, Hoffert KA, Petkov SG et al. Perinatal physiology in cloned and normal calves: Hematologic and biochemical profiles. Cloning and Stem Cells. 2007;9(1):83-96. https://doi.org/10.1089/clo.2006.0038
Batchelder, Cynthia A. ; Bertolini, Marcelo ; Mason, Jeffrey B. ; Moyer, Alice L. ; Hoffert, Kara A. ; Petkov, Stoyan G. ; Famula, Thomas R. ; Angelos, John A ; George, Lisle W. ; Anderson, Gary B. / Perinatal physiology in cloned and normal calves : Hematologic and biochemical profiles. In: Cloning and Stem Cells. 2007 ; Vol. 9, No. 1. pp. 83-96.
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