Perinatal exposure to aged and diluted sidestream cigarette smoke produces airway hyperresponsiveness in older rats

Jesse P. Joad, John M. Bric, Janice L. Peake, Kent E Pinkerton

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Exposing rats to aged and diluted sidestream cigarette smoke (ADSS) throughout in utero and postnatal life results in airway hyperresponsiveness and an increase in pulmonary neuroendocrine cells (PNECs) and neuroepithelial bodies (NEBs) in 7- to 10-week-old rats. Since human epidemiologic studies suggest that perinatal exposure to environmental tobacco smoke (ETS) may be detrimental to the lung function of older children, this study was designed to determine if perinatal exposure alone results in airway hyperresponsiveness and increased PNECs/NEBs later in life in rats. Pregnant Sprague-Dawley rats were exposed to filtered air (FA, n = 7) or ADSS (1 mg/m3 total suspended particulates, n = 7) for 4 to 6 h/day starting on Day 3 of gestation. Their pups continued to receive the same exposure regimen postnatally until 21 days of age. Thereafter all pups were exposed to FA until about 8 weeks of age. The airway responsiveness of one female pup from each litter was then assessed using an isolated perfused lung system whereby increasing doses of methacholine (-9.25 to -7.50 log mol) were administered into the pulmonary artery and lung resistance (RL), dynamic compliance (Cdyn), and pulmonary pressure (Ppa) were measured. The number of PNECs/NEBs and mast cells per millimeter basal lamina were determined using immunocytochemical and histological staining and morphometric analysis. Statistics were performed using an unpaired Student's t test and repeated measures analysis of variance. Perinatal ADSS exposure enhanced methacholine- induced changes in RL (p = 0.02), Cdyn (p = 0.004), and Ppa (p = 0.007). At the highest dose of methacholine, RL in the ADSS-exposed lungs was threefold that in FA-exposed lungs. Although total PNEC number increased approximately twofold in the ADSS-exposed animals, this change was not found to be statistically significant. Mast cell number also was not different between groups. These data suggest that exposure to ADSS during the perinatal period followed by 5 weeks exposure to FA induces airway hyperresponsiveness in the absence of a significant change in PNECs, NEBs, or mast cells.

Original languageEnglish (US)
Pages (from-to)253-260
Number of pages8
JournalToxicology and Applied Pharmacology
Volume155
Issue number3
DOIs
StatePublished - Mar 15 1999

Fingerprint

Smoke
Tobacco Products
Rats
Lung
Neuroepithelial Bodies
Neuroendocrine Cells
Methacholine Chloride
Mast Cells
Neuroepithelial Cells
Tobacco
Analysis of variance (ANOVA)
Cell Count
Animals
Statistics
Environmental Exposure
Students
Basement Membrane
Pulmonary Artery
Compliance
Sprague Dawley Rats

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Perinatal exposure to aged and diluted sidestream cigarette smoke produces airway hyperresponsiveness in older rats. / Joad, Jesse P.; Bric, John M.; Peake, Janice L.; Pinkerton, Kent E.

In: Toxicology and Applied Pharmacology, Vol. 155, No. 3, 15.03.1999, p. 253-260.

Research output: Contribution to journalArticle

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