Performance of two immunoassays for the determination of atrazine in sea water samples as compared with on-line solid phase extraction-liquid chromatography-diode array detection

Jordi Gascón, Anna Oubiña, Imma Ferrer, Patrik Önnerfjord, Gyorgy Marko-Varga, Bruce D. Hammock, Maria Pilar Marco, Damià Barceló

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Two immunoassay formats, magnetic particles-based assay (Atrazine RaPID assay and Atrazine High-Sensitivity RaPID assay) and microtiter plate based assay (Department of Entomology and Environmental Toxicology, University of California in Davis) were evaluated for the determination of atrazine in sea water samples. The results obtained were compared and validated with those obtained by using on-line solid phase extraction followed by liquid chromatography-diode array detection (on-line SPE-LC-DAD). The correlation between both techniques was good when analyzing levels of atrazine ranging from 0.01 to 5 μg/l in samples showing salt concentration values varying from 0 to 35 g/l and pH values from 2 to 10. One of these immunoassays (Atrazine High-Sensitivity RaPID assay) was employed to directly analyze atrazine in real estuarine and coastal water samples. The same samples were analyzed after filtration and C18 Empore disks extraction.

Original languageEnglish (US)
Pages (from-to)41-51
Number of pages11
JournalAnalytica Chimica Acta
Volume330
Issue number1
DOIs
StatePublished - Aug 30 1996

Keywords

  • Atrazine
  • Chromatography
  • Immunoassay
  • Sea water
  • Solid-phase extraction
  • Waters

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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