Performance of a combined optical coherence tomography and scanning laser ophthalmoscope with adaptive optics for human retinal imaging applications

Elaine M. Wells-Gray, Robert Zawadzki, Susanna C. Finn, Cherry Greiner, John S Werner, Stacey S. Choi, Nathan Doble

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We describe the design and performance of a recently implemented retinal imaging system for the human eye that combines adaptive optics (AO) with spectral domain optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO). The AO-OCT-SLO system simultaneously acquires SLO frames and OCT B-scans at 60 Hz with an OCT volume acquisition scan rate of 0.24 Hz. The SLO images are used to correct for eye motion during the registration of OCT B-scans. Key optical design considerations are discussed including: minimizing system aberrations through the use of off-axis relay telescopes; choice of telescope magnification based on pupil plane requirements and restrictions; and the use of dichroic beam splitters to separate and re-combine OCT and SLO beams around the nonshared horizontal scanning mirrors. We include an analysis of closed-loop AO correction on a model eye and compare these findings with system performance in vivo. The 2D and 3D OCT scans included in this work demonstrate the ability of this system to laterally and axially resolve individual cone photoreceptors, while the corresponding SLO images show the en face mosaics at the photoreceptor layer showing rods and cones. Images from both healthy and diseased retina are presented.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9335
ISBN (Print)9781628414257
DOIs
StatePublished - 2015
EventAdaptive Optics and Wavefront Control for Biological Systems - San Francisco, United States
Duration: Feb 7 2015Feb 9 2015

Other

OtherAdaptive Optics and Wavefront Control for Biological Systems
CountryUnited States
CitySan Francisco
Period2/7/152/9/15

Fingerprint

Ophthalmoscopes
Adaptive optics
Optical tomography
Ophthalmoscopy
Optical Coherence Tomography
adaptive optics
Lasers
tomography
Scanning
Imaging techniques
scanning
lasers
Telescopes
photoreceptors
Cones
cones
telescopes
Retinal Cone Photoreceptor Cells
Vertebrate Photoreceptor Cells
Optical design

Keywords

  • Adaptive optics
  • Cones
  • Optical coherence tomography
  • Photoreceptors
  • Retina
  • Rods
  • Scanning Laser Ophthalmoscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Wells-Gray, E. M., Zawadzki, R., Finn, S. C., Greiner, C., Werner, J. S., Choi, S. S., & Doble, N. (2015). Performance of a combined optical coherence tomography and scanning laser ophthalmoscope with adaptive optics for human retinal imaging applications. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9335). [93350N] SPIE. https://doi.org/10.1117/12.2079772

Performance of a combined optical coherence tomography and scanning laser ophthalmoscope with adaptive optics for human retinal imaging applications. / Wells-Gray, Elaine M.; Zawadzki, Robert; Finn, Susanna C.; Greiner, Cherry; Werner, John S; Choi, Stacey S.; Doble, Nathan.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9335 SPIE, 2015. 93350N.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wells-Gray, EM, Zawadzki, R, Finn, SC, Greiner, C, Werner, JS, Choi, SS & Doble, N 2015, Performance of a combined optical coherence tomography and scanning laser ophthalmoscope with adaptive optics for human retinal imaging applications. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9335, 93350N, SPIE, Adaptive Optics and Wavefront Control for Biological Systems, San Francisco, United States, 2/7/15. https://doi.org/10.1117/12.2079772
Wells-Gray EM, Zawadzki R, Finn SC, Greiner C, Werner JS, Choi SS et al. Performance of a combined optical coherence tomography and scanning laser ophthalmoscope with adaptive optics for human retinal imaging applications. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9335. SPIE. 2015. 93350N https://doi.org/10.1117/12.2079772
Wells-Gray, Elaine M. ; Zawadzki, Robert ; Finn, Susanna C. ; Greiner, Cherry ; Werner, John S ; Choi, Stacey S. ; Doble, Nathan. / Performance of a combined optical coherence tomography and scanning laser ophthalmoscope with adaptive optics for human retinal imaging applications. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9335 SPIE, 2015.
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