Abstract
The design of fiber-optic probes plays an important role in optical spectroscopic studies, including fluorescence spectroscopy of biological tissues. It can affect the light delivery and propagation into the tissue, the collection efficiency (total number of photons collected vs. total number of photons launched) and the origin of collected light. This in turn affects the signal to noise ratio (SNR) and the extend of tissue interrogation, thus influencing the diagnostic value of such techniques. Three specific fiber-optic probe designs were tested both experimentally and computationally via Monte Carlo simulations. In particular, the effects of probe architecture (single-fiber vs. two bifurcated multifiber probes), probe-to-target distance (PTD), and source-to-detector separation (SDS) were investigated on the collected diffuse reflectance of a Lambertian target and an agar-based tissue phantom. This study demonstrated that probe architecture, PTD, and SDS are closely intertwined and considerably affect the light collection efficiency, the extend of target illumination, and the origin of the collected reflected light. Our findings can be applied towards optimization of fiber-optic probe designs for quantitative fluorescence spectroscopy of diseased tissues.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | T. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn |
| Pages | 43-50 |
| Number of pages | 8 |
| Volume | 4958 |
| DOIs | |
| State | Published - 2003 |
| Event | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems - San Jose, CA, United States Duration: Jan 26 2003 → Jan 28 2003 |
Other
| Other | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/26/03 → 1/28/03 |
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Keywords
- Diffuse reflectance
- Fiberoptic probes
- Fluorescence spectroscopy
- Optical diagnostics
- Optical properties
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy. / Papaioannou, Thanassis; Preyer, Norris; Fang, Qiyin; Kurt, Hamza; Carnohan, Michael; Ross, Russel; Brightwell, Adam; Cottone, Greg; Jones, Linda; Marcu, Laura.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / T. Vo-Dinh; W.S. Grundfest; D.A. Benaron; G.E. Cohn. Vol. 4958 2003. p. 43-50.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy
AU - Papaioannou, Thanassis
AU - Preyer, Norris
AU - Fang, Qiyin
AU - Kurt, Hamza
AU - Carnohan, Michael
AU - Ross, Russel
AU - Brightwell, Adam
AU - Cottone, Greg
AU - Jones, Linda
AU - Marcu, Laura
PY - 2003
Y1 - 2003
N2 - The design of fiber-optic probes plays an important role in optical spectroscopic studies, including fluorescence spectroscopy of biological tissues. It can affect the light delivery and propagation into the tissue, the collection efficiency (total number of photons collected vs. total number of photons launched) and the origin of collected light. This in turn affects the signal to noise ratio (SNR) and the extend of tissue interrogation, thus influencing the diagnostic value of such techniques. Three specific fiber-optic probe designs were tested both experimentally and computationally via Monte Carlo simulations. In particular, the effects of probe architecture (single-fiber vs. two bifurcated multifiber probes), probe-to-target distance (PTD), and source-to-detector separation (SDS) were investigated on the collected diffuse reflectance of a Lambertian target and an agar-based tissue phantom. This study demonstrated that probe architecture, PTD, and SDS are closely intertwined and considerably affect the light collection efficiency, the extend of target illumination, and the origin of the collected reflected light. Our findings can be applied towards optimization of fiber-optic probe designs for quantitative fluorescence spectroscopy of diseased tissues.
AB - The design of fiber-optic probes plays an important role in optical spectroscopic studies, including fluorescence spectroscopy of biological tissues. It can affect the light delivery and propagation into the tissue, the collection efficiency (total number of photons collected vs. total number of photons launched) and the origin of collected light. This in turn affects the signal to noise ratio (SNR) and the extend of tissue interrogation, thus influencing the diagnostic value of such techniques. Three specific fiber-optic probe designs were tested both experimentally and computationally via Monte Carlo simulations. In particular, the effects of probe architecture (single-fiber vs. two bifurcated multifiber probes), probe-to-target distance (PTD), and source-to-detector separation (SDS) were investigated on the collected diffuse reflectance of a Lambertian target and an agar-based tissue phantom. This study demonstrated that probe architecture, PTD, and SDS are closely intertwined and considerably affect the light collection efficiency, the extend of target illumination, and the origin of the collected reflected light. Our findings can be applied towards optimization of fiber-optic probe designs for quantitative fluorescence spectroscopy of diseased tissues.
KW - Diffuse reflectance
KW - Fiberoptic probes
KW - Fluorescence spectroscopy
KW - Optical diagnostics
KW - Optical properties
UR - http://www.scopus.com/inward/record.url?scp=0345714612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345714612&partnerID=8YFLogxK
U2 - 10.1117/12.476141
DO - 10.1117/12.476141
M3 - Conference contribution
AN - SCOPUS:0345714612
VL - 4958
SP - 43
EP - 50
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Vo-Dinh, T.
A2 - Grundfest, W.S.
A2 - Benaron, D.A.
A2 - Cohn, G.E.
ER -