Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy

Thanassis Papaioannou, Norris Preyer, Qiyin Fang, Hamza Kurt, Michael Carnohan, Russel Ross, Adam Brightwell, Greg Cottone, Linda Jones, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations

Abstract

The design of fiber-optic probes plays an important role in optical spectroscopic studies, including fluorescence spectroscopy of biological tissues. It can affect the light delivery and propagation into the tissue, the collection efficiency (total number of photons collected vs. total number of photons launched) and the origin of collected light. This in turn affects the signal to noise ratio (SNR) and the extend of tissue interrogation, thus influencing the diagnostic value of such techniques. Three specific fiber-optic probe designs were tested both experimentally and computationally via Monte Carlo simulations. In particular, the effects of probe architecture (single-fiber vs. two bifurcated multifiber probes), probe-to-target distance (PTD), and source-to-detector separation (SDS) were investigated on the collected diffuse reflectance of a Lambertian target and an agar-based tissue phantom. This study demonstrated that probe architecture, PTD, and SDS are closely intertwined and considerably affect the light collection efficiency, the extend of target illumination, and the origin of the collected reflected light. Our findings can be applied towards optimization of fiber-optic probe designs for quantitative fluorescence spectroscopy of diseased tissues.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsT. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn
Pages43-50
Number of pages8
Volume4958
DOIs
StatePublished - 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems - San Jose, CA, United States
Duration: Jan 26 2003Jan 28 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Advanced Biomedical and Clinical Diagnostic Systems
CountryUnited States
CitySan Jose, CA
Period1/26/031/28/03

Keywords

  • Diffuse reflectance
  • Fiberoptic probes
  • Fluorescence spectroscopy
  • Optical diagnostics
  • Optical properties

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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  • Cite this

    Papaioannou, T., Preyer, N., Fang, Q., Kurt, H., Carnohan, M., Ross, R., Brightwell, A., Cottone, G., Jones, L., & Marcu, L. (2003). Performance evaluation of fiber optic probes for tissue lifetime fluorescence spectroscopy. In T. Vo-Dinh, W. S. Grundfest, D. A. Benaron, & G. E. Cohn (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4958, pp. 43-50) https://doi.org/10.1117/12.476141