Perfluorocarbon-associated gas exchange improves oxygenation, lung mechanics, and survival in a model of adult respiratory distress syndrome

Michele C. Papo, Pamela R. Paczan, Bradley P. Fuhrman, David M. Steinhorn, Lynn J. Hernan, Corinne L. Leach, Bruce A. Holm, John E. Fisher, Beverly A. Kahn

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Objective: To compare the effectiveness of perfluorocarbon-associated gas exchange to volume controlled positive pressure breathing in supporting gas exchange, lung mechanics, and survival in an acute lung injury model. Design: A prospective, randomized study. Setting: A university medical school laboratory approved for animal research. Subjects: Neonatal piglets. Interventions: Eighteen piglets were randomized to receive perfluorocarbon- associated gas exchange with perflubron (n = 10) or volume controlled continuous positive pressure breathing (n = 8) after acute lung injury was induced by oleic acid infusion (0.15 mL/kg iv). Measurements and Main Results: Arterial and venous blood gases, hemodynamics, and lung mechanics were measured every 15 mins during a 3-hr study period. All animals developed a metabolic and a respiratory acidosis during the infusion of oleic acid. Following randomization, the volume controlled positive pressure breathing group developed a profound acidosis (p < .05), while pH did not change in the perfluorocarbon-associated gas exchange group. Within 16 mins of initiating perfluorocarbon-associated gas exchange, oxygenation increased from a PaO2 of 52 ± 12 torr (6.92 ± 1.60 kPa) to 161 ± 93 torr (20.0 ± 12.4 kPa) and continued to improve throughout the study (p < .05). Animals that received volume controlled positive pressure breathing remained hypoxic with no appreciable change in PaO2. Although both groups developed hypercarbia during oleic acid infusion, PaCO2 steadily increased over time in the control group (p < .01). Static lung compliance significantly increased postrandomization (60 mins) in the animals supported by perfluorocarbon-associated gas exchange (p < .05), whereas it remained unchanged over time in the volume controlled positive pressure breathing group. However, survival was significantly higher in the perfluorocarbon- associated gas exchange group with eight (60%) of ten animals surviving the entire study period. Only two (25%) of the eight animals in the volume controlled positive pressure breathing group were alive at the end of the study period (log-rank statistic, p = .013). Conclusions: Perfluorocarbon- associated gas exchange enhanced gas exchange, pulmonary mechanics, and survival in this model of acute lung injury.

Original languageEnglish (US)
Pages (from-to)466-474
Number of pages9
JournalCritical Care Medicine
Volume24
Issue number3
DOIs
StatePublished - Mar 1996
Externally publishedYes

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Fluorocarbons
Adult Respiratory Distress Syndrome
Mechanics
Gases
Lung
Respiration
Pressure
Acute Lung Injury
Oleic Acid
Respiratory Acidosis
Pulmonary Gas Exchange
Lung Compliance
Hypercapnia
Laboratory Animals
Random Allocation
Acidosis
Medical Schools
Hemodynamics
Prospective Studies
Control Groups

Keywords

  • acute lung injury
  • adult respiratory distress syndrome
  • liquid ventilation
  • lung compliance
  • mechanical ventilation
  • oleic acid
  • perfluorocarbons
  • pulmonary edema

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Perfluorocarbon-associated gas exchange improves oxygenation, lung mechanics, and survival in a model of adult respiratory distress syndrome. / Papo, Michele C.; Paczan, Pamela R.; Fuhrman, Bradley P.; Steinhorn, David M.; Hernan, Lynn J.; Leach, Corinne L.; Holm, Bruce A.; Fisher, John E.; Kahn, Beverly A.

In: Critical Care Medicine, Vol. 24, No. 3, 03.1996, p. 466-474.

Research output: Contribution to journalArticle

Papo, MC, Paczan, PR, Fuhrman, BP, Steinhorn, DM, Hernan, LJ, Leach, CL, Holm, BA, Fisher, JE & Kahn, BA 1996, 'Perfluorocarbon-associated gas exchange improves oxygenation, lung mechanics, and survival in a model of adult respiratory distress syndrome', Critical Care Medicine, vol. 24, no. 3, pp. 466-474. https://doi.org/10.1097/00003246-199603000-00017
Papo, Michele C. ; Paczan, Pamela R. ; Fuhrman, Bradley P. ; Steinhorn, David M. ; Hernan, Lynn J. ; Leach, Corinne L. ; Holm, Bruce A. ; Fisher, John E. ; Kahn, Beverly A. / Perfluorocarbon-associated gas exchange improves oxygenation, lung mechanics, and survival in a model of adult respiratory distress syndrome. In: Critical Care Medicine. 1996 ; Vol. 24, No. 3. pp. 466-474.
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AU - Paczan, Pamela R.

AU - Fuhrman, Bradley P.

AU - Steinhorn, David M.

AU - Hernan, Lynn J.

AU - Leach, Corinne L.

AU - Holm, Bruce A.

AU - Fisher, John E.

AU - Kahn, Beverly A.

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N2 - Objective: To compare the effectiveness of perfluorocarbon-associated gas exchange to volume controlled positive pressure breathing in supporting gas exchange, lung mechanics, and survival in an acute lung injury model. Design: A prospective, randomized study. Setting: A university medical school laboratory approved for animal research. Subjects: Neonatal piglets. Interventions: Eighteen piglets were randomized to receive perfluorocarbon- associated gas exchange with perflubron (n = 10) or volume controlled continuous positive pressure breathing (n = 8) after acute lung injury was induced by oleic acid infusion (0.15 mL/kg iv). Measurements and Main Results: Arterial and venous blood gases, hemodynamics, and lung mechanics were measured every 15 mins during a 3-hr study period. All animals developed a metabolic and a respiratory acidosis during the infusion of oleic acid. Following randomization, the volume controlled positive pressure breathing group developed a profound acidosis (p < .05), while pH did not change in the perfluorocarbon-associated gas exchange group. Within 16 mins of initiating perfluorocarbon-associated gas exchange, oxygenation increased from a PaO2 of 52 ± 12 torr (6.92 ± 1.60 kPa) to 161 ± 93 torr (20.0 ± 12.4 kPa) and continued to improve throughout the study (p < .05). Animals that received volume controlled positive pressure breathing remained hypoxic with no appreciable change in PaO2. Although both groups developed hypercarbia during oleic acid infusion, PaCO2 steadily increased over time in the control group (p < .01). Static lung compliance significantly increased postrandomization (60 mins) in the animals supported by perfluorocarbon-associated gas exchange (p < .05), whereas it remained unchanged over time in the volume controlled positive pressure breathing group. However, survival was significantly higher in the perfluorocarbon- associated gas exchange group with eight (60%) of ten animals surviving the entire study period. Only two (25%) of the eight animals in the volume controlled positive pressure breathing group were alive at the end of the study period (log-rank statistic, p = .013). Conclusions: Perfluorocarbon- associated gas exchange enhanced gas exchange, pulmonary mechanics, and survival in this model of acute lung injury.

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KW - acute lung injury

KW - adult respiratory distress syndrome

KW - liquid ventilation

KW - lung compliance

KW - mechanical ventilation

KW - oleic acid

KW - perfluorocarbons

KW - pulmonary edema

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