Percutaneous fiber-optic sensor for chronic glucose monitoring in vivo

Kuo Chih Liao, Thieo Hogen-Esch, Frances J. Richmond, Laura Marcu, William Clifton, Gerald E. Loeb

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

We are developing a family of fiber-optic sensors called Sencils™ (sensory cilia), which are disposable, minimally invasive, and can provide in vivo monitoring of various analytes for several weeks. The key element is a percutaneous optical fiber that permits reliable spectroscopic measurement of chemical reactions in a nano-engineered polymeric matrix attached to the implanted end of the fiber. This paper describes its first application to measure interstitial glucose based on changes in fluorescence resonance energy transfer (FRET) between fluorophores bound to betacyclodextrin and Concanavalin A (Con A) in a polyethylene glycol (PEG) matrix. In vitro experiments demonstrate a rapid and precise relationship between the ratio of the two fluorescent emissions and concentration of glucose in saline for the physiological range of concentrations (0-500 mg/dl) over seven weeks. Chronic animal implantation studies have demonstrated good biocompatibility and durability for clinical applications.

Original languageEnglish (US)
Pages (from-to)1458-1465
Number of pages8
JournalBiosensors and Bioelectronics
Volume23
Issue number10
DOIs
StatePublished - May 15 2008
Externally publishedYes

Keywords

  • Affinity-binding assay
  • Chemical sensor
  • Fluorescence resonance energy transfer
  • Glucose
  • Optical fiber
  • Quantum dots

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Fingerprint Dive into the research topics of 'Percutaneous fiber-optic sensor for chronic glucose monitoring in vivo'. Together they form a unique fingerprint.

  • Cite this

    Liao, K. C., Hogen-Esch, T., Richmond, F. J., Marcu, L., Clifton, W., & Loeb, G. E. (2008). Percutaneous fiber-optic sensor for chronic glucose monitoring in vivo. Biosensors and Bioelectronics, 23(10), 1458-1465. https://doi.org/10.1016/j.bios.2008.01.012