Peptidolytic monoclonal antibody elicited by a neuropeptide

Sudhir Paul, Mei Sun, Rustom Mody, Hemant K. Tewary, Paul Stemmer, Richard J. Massey, Teresa Gianferrara, Sanjiv Mehrotra, Thomas Dreyer, Morton Meldal, Alfonso Tramontano

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Abstract

We report evidence that a monoclonal antibody raised by immunization with a vasoactive intestinal peptide (VIP)-carrier protein conjugate selectively hydrolyzes VIP and a fluorescence quenched decapeptide (FQ14-22D), representing the region of VIP most susceptible to autoantibody-mediated cleavage (residues 14-22). A high affinity of the antibody for VIP and a lower affinity for FQ14-22D were revealed by kinetic studies and further substantiated by potent inhibition of FQ14-22D cleaving activity by full-length VIP. Sequencing of FQ14-22D hydrolysis products indicated selective cleavage at one peptide bond. These observations suggest that antibodies induced against naturally occurring polypeptide antigens can express peptidolytic activity targeted for specific sequences in the recognition epitope.

Original languageEnglish (US)
Pages (from-to)13142-13145
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number19
StatePublished - Jul 5 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Paul, S., Sun, M., Mody, R., Tewary, H. K., Stemmer, P., Massey, R. J., Gianferrara, T., Mehrotra, S., Dreyer, T., Meldal, M., & Tramontano, A. (1992). Peptidolytic monoclonal antibody elicited by a neuropeptide. Journal of Biological Chemistry, 267(19), 13142-13145.