Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells.

Nitin Nitin, Philip J. Santangelo, Gloria Kim, Shuming Nie, Gang Bao

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Real-time visualization of specific endogenous mRNA expression in vivo has the potential to revolutionize medical diagnosis, drug discovery, developmental and molecular biology. However, conventional liposome- or dendrimer-based cellular delivery of molecular probes is inefficient, slow, and often detrimental to the probes. Here we demonstrate the rapid and sensitive detection of RNA in living cells using peptide-linked molecular beacons that possess self-delivery, targeting and reporting functions. We conjugated the TAT peptide to molecular beacons using three different linkages and demonstrated that, at relatively low concentrations, these molecular beacon constructs were internalized into living cells within 30 min with nearly 100% efficiency. Further, peptide-based delivery did not interfere with either specific targeting by or hybridization-induced fluorescence of the probes. We could therefore detect human GAPDH and survivin mRNAs in living cells fluorescently, revealing intriguing intracellular localization patterns of mRNA. We clearly demonstrated that cellular delivery of molecular beacons using the peptide-based approach has far better performance compared with conventional transfection methods. The peptide-linked molecular beacons approach promises to open new and exciting opportunities in sensitive gene detection and quantification in vivo.

Original languageEnglish (US)
JournalNucleic Acids Research
Volume32
Issue number6
StatePublished - Mar 2004
Externally publishedYes

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Messenger RNA
Peptides
Dendrimers
Molecular Probes
Developmental Biology
Drug Discovery
Liposomes
Transfection
Molecular Biology
Fluorescence
RNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells. / Nitin, Nitin; Santangelo, Philip J.; Kim, Gloria; Nie, Shuming; Bao, Gang.

In: Nucleic Acids Research, Vol. 32, No. 6, 03.2004.

Research output: Contribution to journalArticle

Nitin, Nitin ; Santangelo, Philip J. ; Kim, Gloria ; Nie, Shuming ; Bao, Gang. / Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells. In: Nucleic Acids Research. 2004 ; Vol. 32, No. 6.
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