Abstract
Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
Original language | English (US) |
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Pages (from-to) | 217-226 |
Number of pages | 10 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 310 |
State | Published - 2005 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics