Abstract
Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-226 |
| Number of pages | 10 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 310 |
| State | Published - 2005 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Peptide and small-molecule microarrays. / Marik, Jan; Lam, Kit.
In: Methods in molecular biology (Clifton, N.J.), Vol. 310, 2005, p. 217-226.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peptide and small-molecule microarrays.
AU - Marik, Jan
AU - Lam, Kit
PY - 2005
Y1 - 2005
N2 - Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
AB - Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
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UR - http://www.scopus.com/inward/citedby.url?scp=33644815190&partnerID=8YFLogxK
M3 - Article
C2 - 16353378
AN - SCOPUS:33644815190
VL - 310
SP - 217
EP - 226
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -