Peptide and small-molecule microarrays.

Jan Marik, Kit Lam

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.

Original languageEnglish (US)
Pages (from-to)217-226
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume310
StatePublished - 2005

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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