Peptide and small-molecule microarrays.

Jan Marik, Kit Lam

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.

Original languageEnglish (US)
Pages (from-to)217-226
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume310
StatePublished - 2005

Fingerprint

Peptides
Ligation
Glass
Ligands

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Peptide and small-molecule microarrays. / Marik, Jan; Lam, Kit.

In: Methods in molecular biology (Clifton, N.J.), Vol. 310, 2005, p. 217-226.

Research output: Contribution to journalArticle

@article{625acbe3d5c444ddb128c849b5b84b41,
title = "Peptide and small-molecule microarrays.",
abstract = "Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.",
author = "Jan Marik and Kit Lam",
year = "2005",
language = "English (US)",
volume = "310",
pages = "217--226",
journal = "Methods in molecular biology (Clifton, N.J.)",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Peptide and small-molecule microarrays.

AU - Marik, Jan

AU - Lam, Kit

PY - 2005

Y1 - 2005

N2 - Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.

AB - Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.

UR - http://www.scopus.com/inward/record.url?scp=33644815190&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644815190&partnerID=8YFLogxK

M3 - Article

C2 - 16353378

AN - SCOPUS:33644815190

VL - 310

SP - 217

EP - 226

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

SN - 1064-3745

ER -