Peptide amidating activity in human bronchoalveolar lavage fluid

Frank M. Scott, Anthony M. Treston, Gail L. Shaw, Ingalill Avis, Julie Sorenson, Karen Kelly, Edward C. Dempsey, Alan B. Cantor, Melvyn Tockman, James L. Mulshine

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Monitoring respiratory epithelial biology may reveal individuals with incipient lung cancer. The expression of neuroendocrine (NE) markers in pulmonary epithelium is thought to be central to lung development, repair of injury and may contribute to carcinogenesis. In this study, we evaluate several candidate NE markers to determine the feasibility of prospective analysis of clinical specimens. The potential NE markers include the enzyme L-DOPA decarboxylase (DDC), the neuropeptide gastrin releasing peptide (GRP), and peptidyl-glycine α-amidating monooxygenase (PAM), the bifunctional enzyme responsible for the final bioactivation step of many neuropeptides. A comparison of PAM activity and DDC levels in 30 lung cancer cell lines indicated that peptide amidating activity may be an indicator of NE status. Bronchoalveolar lavage (BAL) fluid from subjects at risk of developing second primary lung cancer and from volunteers was obtained. The activity of the first PAM enzyme, peptidylglycine α-hydroxylating monooxygenase (PHM), ranged from not detectable to 507 pmol/h/mg protein in 57 specimens. The second PAM enzyme, peptidylamidoglycolate lyase (PAL), ranged from not detectable to 414 pmol/h/mg protein in 56 specimens. Using cluster analysis by the average linkage method, a group of enzyme values with PHM greater than 230 pmol/h/mg protein was determined. Long-term follow-up of these patients for new second primary lung cancers may help to determine the potential predictive value of PAM detected in the BAL fluid.

Original languageEnglish (US)
Pages (from-to)239-251
Number of pages13
JournalLung Cancer
Volume14
Issue number2-3
DOIs
StatePublished - Jun 1996
Externally publishedYes

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Bronchoalveolar Lavage Fluid
Mixed Function Oxygenases
Human Activities
Glycine
Lung Neoplasms
Peptides
Enzymes
Carboxy-Lyases
Second Primary Neoplasms
peptidylamidoglycolate lyase
Neuropeptides
Gastrin-Releasing Peptide
Lung
Proteins
Cluster Analysis
Volunteers
Carcinogenesis
Epithelium
Cell Line
Wounds and Injuries

Keywords

  • Biomarkers
  • Prevention
  • Translational research

ASJC Scopus subject areas

  • Oncology

Cite this

Scott, F. M., Treston, A. M., Shaw, G. L., Avis, I., Sorenson, J., Kelly, K., ... Mulshine, J. L. (1996). Peptide amidating activity in human bronchoalveolar lavage fluid. Lung Cancer, 14(2-3), 239-251. https://doi.org/10.1016/0169-5002(96)00550-8

Peptide amidating activity in human bronchoalveolar lavage fluid. / Scott, Frank M.; Treston, Anthony M.; Shaw, Gail L.; Avis, Ingalill; Sorenson, Julie; Kelly, Karen; Dempsey, Edward C.; Cantor, Alan B.; Tockman, Melvyn; Mulshine, James L.

In: Lung Cancer, Vol. 14, No. 2-3, 06.1996, p. 239-251.

Research output: Contribution to journalArticle

Scott, FM, Treston, AM, Shaw, GL, Avis, I, Sorenson, J, Kelly, K, Dempsey, EC, Cantor, AB, Tockman, M & Mulshine, JL 1996, 'Peptide amidating activity in human bronchoalveolar lavage fluid', Lung Cancer, vol. 14, no. 2-3, pp. 239-251. https://doi.org/10.1016/0169-5002(96)00550-8
Scott FM, Treston AM, Shaw GL, Avis I, Sorenson J, Kelly K et al. Peptide amidating activity in human bronchoalveolar lavage fluid. Lung Cancer. 1996 Jun;14(2-3):239-251. https://doi.org/10.1016/0169-5002(96)00550-8
Scott, Frank M. ; Treston, Anthony M. ; Shaw, Gail L. ; Avis, Ingalill ; Sorenson, Julie ; Kelly, Karen ; Dempsey, Edward C. ; Cantor, Alan B. ; Tockman, Melvyn ; Mulshine, James L. / Peptide amidating activity in human bronchoalveolar lavage fluid. In: Lung Cancer. 1996 ; Vol. 14, No. 2-3. pp. 239-251.
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