Pentamidine-induced alteration in restriction endonuclease cleavage of plasmid DNA

Kanakendu Choudhury, Michael J Leibowitz

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We have used restriction enzymes and DNaseI as probes to determine the specificity of pentamidine binding to plasmid DNA. Cleavage of plasmid pAZ130 by EcoRI, EcoRV and ApaI is inhibited by pentamidine, cleavage by XbaI, NotI and AvaI is unaffected, while cleavage by XhoI, which recognizes the same sequence as AvaI, is stimulated. DNaseI foot-printing of DNA containing these restriction sites revealed that pentamidine protection is not strictly limited to AT-rich regions. We suggest that perturbation of the DNA micro-environment by pentamidine binding is responsible for its effect on nucleases.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalJournal of Biomolecular Structure and Dynamics
Volume21
Issue number1
StatePublished - Aug 2003
Externally publishedYes

Keywords

  • DNA
  • DNaseI
  • Pentamidine
  • Restriction Enzyme

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

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