Pentamidine-induced alteration in restriction endonuclease cleavage of plasmid DNA

Kanakendu Choudhury, Michael J Leibowitz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have used restriction enzymes and DNaseI as probes to determine the specificity of pentamidine binding to plasmid DNA. Cleavage of plasmid pAZ130 by EcoRI, EcoRV and ApaI is inhibited by pentamidine, cleavage by XbaI, NotI and AvaI is unaffected, while cleavage by XhoI, which recognizes the same sequence as AvaI, is stimulated. DNaseI foot-printing of DNA containing these restriction sites revealed that pentamidine protection is not strictly limited to AT-rich regions. We suggest that perturbation of the DNA micro-environment by pentamidine binding is responsible for its effect on nucleases.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalJournal of Biomolecular Structure and Dynamics
Volume21
Issue number1
StatePublished - Aug 2003
Externally publishedYes

Fingerprint

Pentamidine
DNA Cleavage
DNA Restriction Enzymes
Plasmids
DNA
AT Rich Sequence
Printing
Foot
Enzymes

Keywords

  • DNA
  • DNaseI
  • Pentamidine
  • Restriction Enzyme

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Pentamidine-induced alteration in restriction endonuclease cleavage of plasmid DNA. / Choudhury, Kanakendu; Leibowitz, Michael J.

In: Journal of Biomolecular Structure and Dynamics, Vol. 21, No. 1, 08.2003, p. 127-134.

Research output: Contribution to journalArticle

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