PDGF and FGF stimulate wound healing in the genetically diabetic mouse

David G Greenhalgh, Katherine H. Sprugel, Mark J. Murray, Russell Ross

Research output: Contribution to journalArticle

526 Citations (Scopus)

Abstract

To examine the effects of recombinant growth factors in vivo, impaired wound healing was studied in genetically diabetic C57BL/KsJ-db/db mice. Large full-thickness skin wounds made on the backs of these mice exhibited significant delays in the entry of inflammatory cells into the wound, the formation of granulation tissue, and in wound closure when compared to their nondiabetic littermates. Recombinant human platelet-derived growth factor (rPDGFBB, 1 or 10 μg), recombinant human basic fibroblast growth factor (rbFGF, 1 μg), or combinations of both were applied topically to the wounds for 5 to 14 days after wounding. Diabetic mouse wounds treated with rPDGF-BB or rbFGF had many more flbroblasts and capillaries in the wound bed at 10 and 21 days than did wounds treated with the vehicle alone. The animals treated with growth factors also had significantly greater wound closure at 21 days than those treated with the vehicle. Combinations of rPDGF-BB and rbFGF improved all parameters of healing but not to a greater extent than either growth factor alone. The effectiveness of rPDGF-BB and rbFGF suggest that recombinant growth factors may be useful in the treatment of patients with deficient wound repair.

Original languageEnglish (US)
Pages (from-to)1235-1246
Number of pages12
JournalAmerican Journal of Pathology
Volume136
Issue number6
StatePublished - Jun 1990
Externally publishedYes

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Wound Healing
Wounds and Injuries
Intercellular Signaling Peptides and Proteins
Fibroblast Growth Factor 1
Granulation Tissue
Platelet-Derived Growth Factor
Skin
platelet-derived growth factor BB

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

PDGF and FGF stimulate wound healing in the genetically diabetic mouse. / Greenhalgh, David G; Sprugel, Katherine H.; Murray, Mark J.; Ross, Russell.

In: American Journal of Pathology, Vol. 136, No. 6, 06.1990, p. 1235-1246.

Research output: Contribution to journalArticle

Greenhalgh, DG, Sprugel, KH, Murray, MJ & Ross, R 1990, 'PDGF and FGF stimulate wound healing in the genetically diabetic mouse', American Journal of Pathology, vol. 136, no. 6, pp. 1235-1246.
Greenhalgh, David G ; Sprugel, Katherine H. ; Murray, Mark J. ; Ross, Russell. / PDGF and FGF stimulate wound healing in the genetically diabetic mouse. In: American Journal of Pathology. 1990 ; Vol. 136, No. 6. pp. 1235-1246.
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