PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp.

M. Hill, H. Witsenboer, M. Zabeau, P. Vos, R. Kesseli, Richard W Michelmore

Research output: Contribution to journalArticle

177 Scopus citations

Abstract

AFLP markers were evaluated for determining the phylogenetic relationships Lactuca spp. Genetic distances based on AFLP data were estimated for 44 morphologically diverse lines of cultivated L. sativa and 13 accessions of the wild species L. serriola, L. saligna, L. virosa, L. perennis, and L. indica. The same genotypes were analyzed as in a previous study that had utilized RFLP markers. The phenetic tree based on AFLP data was consistent with known taxonomic relationships and similar to a tree developed with RFLP data. The genetic distance matrices derived from AFLP and RFLP data were compared using least squares regression analysis and, for the cultivar data, by principal component analysis. There was also a positive linear relationship between distance estimates based on AFLP data and kinship coefficients calculated from pedigree data. AFLPs represent reliable PCR- based markers for studies of genetic relationships at a variety of taxonomic levels.

Original languageEnglish (US)
Pages (from-to)1202-1210
Number of pages9
JournalTheoretical And Applied Genetics
Volume93
Issue number8
DOIs
StatePublished - Dec 1996

Keywords

  • AFLP marker
  • Lactuca
  • Lettuce
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

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