PCR assay for improved diagnostics of epitheliotropic disease virus (EEDV) in lake trout Salvelinus namaycush

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Epizootic epitheliotropic disease virus (EEDV) has caused catastrophic losses among hatchery-reared juvenile lake trout Salvelinus namaycush since the early 1980s and remains a major impediment to lake trout restoration in the Great Lakes basin of the USA. Although EEDV has been tentatively designated as a herpesvirus based upon morphological criteria, further characterization of the virus and development of improved detection methods have been hampered by the inability to propagate the virus in cell culture. Recently obtained sequence data for a region of the putative terminase gene from EEDV as well as the related Salmonid herpesvirus 1 and 2 have permitted the development of a polymerase chain reaction (PCR) assay for specific detection of EEDV. The new EEDV PCR demonstrated both an excellent analytic sensitivity and specificity and detected viral DNA as present in the skin of lake trout during periods of active viral outbreaks. In addition, EEDV DNA was detected among healthy appearing juveniles and in the ovarian fluids of spawning adults. Here we describe the development and initial validation steps of the EEDV PCR as a replacement for current diagnostic methods that require virus extraction from the skin, partial purification by isopycnic centrifugation, and visualization of negatively-stained virions by electron microscopy.

Original languageEnglish (US)
Pages (from-to)17-24
Number of pages8
JournalDiseases of Aquatic Organisms
Volume84
Issue number1
DOIs
StatePublished - Apr 6 2009

Fingerprint

Salvelinus namaycush
polymerase chain reaction
virus
assay
viruses
lake
assays
Salmonid herpesvirus 1
Salmonid herpesvirus 2
skin
DNA viruses
DNA
Great Lakes
virion
salmonid
diagnostic techniques
detection method
centrifugation
hatcheries
electron microscopy

Keywords

  • EEDV
  • Fish herpesvirus
  • Lake trout
  • Salmonid herpesvirus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

PCR assay for improved diagnostics of epitheliotropic disease virus (EEDV) in lake trout Salvelinus namaycush. / Kurobe, Tomofumi; Marcquenski, S.; Hedrick, Ronald.

In: Diseases of Aquatic Organisms, Vol. 84, No. 1, 06.04.2009, p. 17-24.

Research output: Contribution to journalArticle

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