Pch2 acts through Xrs2 and Tel1/ATM to modulate interhomolog bias and checkpoint function during meiosis

Hsuan Chung Ho, Sean M. Burgess

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Proper segregation of chromosomes during meiosis requires the formation and repair of double-strand breaks (DSBs) to form crossovers. Repair is biased toward using the homolog as a substrate rather than the sister chromatid. Pch2 is a conserved member of the AAA +-ATPase family of proteins and is implicated in a wide range of meiosis-specific processes including the recombination checkpoint, maturation of the chromosome axis, crossover control, and synapsis. We demonstrate a role for Pch2 in promoting and regulating interhomolog bias and the meiotic recombination checkpoint in response to unprocessed DSBs through the activation of axial proteins Hop1 and Mek1 in budding yeast. We show that Pch2 physically interacts with the putative BRCT repeats in the N-terminal region of Xrs2, a member of the MRX complex that acts at sites of unprocessed DSBs. Pch2, Xrs2, and the ATM ortholog Tel1 function in the same pathway leading to the phosphorylation of Hop1, independent of Rad17 and the ATR ortholog Mec1, which respond to the presence of single-stranded DNA. An N-terminal deletion of Xrs2 recapitulates the pch2Δ phenotypes for signaling unresected breaks. We propose that interaction with Xrs2 may enable Pch2 to remodel chromosome structure adjacent to the site of a DSB and thereby promote accessibility of Hop1 to the Tel1 kinase. In addition, Xrs2, like Pch2, is required for checkpoint-mediated delay conferred by the failure to synapse chromosomes.

Original languageEnglish (US)
Article numbere1002351
JournalPLoS Genetics
Volume7
Issue number11
DOIs
StatePublished - Nov 2011

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airborne thematic mapper
Meiosis
meiosis
Genetic Recombination
chromosome
Chromosomes
Chromosome Structures
chromosomes
Chromosome Pairing
Chromosome Segregation
single-stranded DNA
chromosome pairing
Saccharomycetales
Chromatids
chromosome segregation
chromatids
Single-Stranded DNA
Synapses
recombination
repair

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Pch2 acts through Xrs2 and Tel1/ATM to modulate interhomolog bias and checkpoint function during meiosis. / Ho, Hsuan Chung; Burgess, Sean M.

In: PLoS Genetics, Vol. 7, No. 11, e1002351, 11.2011.

Research output: Contribution to journalArticle

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