Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons

Olga Golonzhka, Alexander Nord, Paul L.F. Tang, Susan Lindtner, Athena R. Ypsilanti, Elisabetta Ferretti, Axel Visel, Licia Selleri, John L.R. Rubenstein

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Abstract

We demonstrate using conditional mutagenesis that Pbx1, with and without Pbx2+/- sensitization, regulates regional identity and laminar patterning of the developing mouse neocortex in cortical progenitors (Emx1-Cre) and in newly generated neurons (Nex1-Cre). Pbx1/2 mutants have three salient molecular phenotypes of cortical regional and laminar organization: hypoplasia of the frontal cortex, ventral expansion of the dorsomedial cortex, and ventral expansion of Reelin expression in the cortical plate of the frontal cortex, concomitant with an inversion of cortical layering in the rostral cortex. Molecular analyses, including PBX ChIP-seq, provide evidence that PBX promotes frontal cortex identity by repressing genes that promote dorsocaudal fate.

Original languageEnglish (US)
Pages (from-to)1192-1207
Number of pages16
JournalNeuron
Volume88
Issue number6
DOIs
StatePublished - Jan 1 2015

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Golonzhka, O., Nord, A., Tang, P. L. F., Lindtner, S., Ypsilanti, A. R., Ferretti, E., Visel, A., Selleri, L., & Rubenstein, J. L. R. (2015). Pbx Regulates Patterning of the Cerebral Cortex in Progenitors and Postmitotic Neurons. Neuron, 88(6), 1192-1207. https://doi.org/10.1016/j.neuron.2015.10.045