Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potential

Charlotte A. Berkes, Donald A. Bergstrom, Bennett Penn, Karen J. Seaver, Paul S Knoepfler, Stephen J. Tapscott

Research output: Contribution to journalArticle

242 Citations (Scopus)

Abstract

Skeletal muscle differentiation is initiated by the transcription factor MyoD, which binds directly to the regulatory regions of genes expressed during skeletal muscle differentiation and initiates chromatin remodeling at specific promoters. It is not known, however, how MyoD initially recognizes its binding site in a chromatin context. Here we show that the H/C and helix III domains, two domains of MyoD that are necessary for the initiation of chromatin remodeling at the myogenin locus, together regulate a restricted subset of genes, including myogenin. These domains are necessary for the stable binding of MyoD to the myogenin promoter through an interaction with an adjacent protein complex containing the homeodomain protein Pbx, which appears to be constitutively bound at this site. This demonstrates a specific mechanism of targeting MyoD to loci in inactive chromatin and reveals a critical role of homeodomain proteins in marking specific genes for activation in the muscle lineage.

Original languageEnglish (US)
Pages (from-to)465-477
Number of pages13
JournalMolecular Cell
Volume14
Issue number4
DOIs
StatePublished - May 21 2004
Externally publishedYes

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Myogenin
Homeodomain Proteins
Transcriptional Activation
Chromatin Assembly and Disassembly
Chromatin
Skeletal Muscle
Nucleic Acid Regulatory Sequences
Regulator Genes
Transcription Factors
Binding Sites
Muscles
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potential. / Berkes, Charlotte A.; Bergstrom, Donald A.; Penn, Bennett; Seaver, Karen J.; Knoepfler, Paul S; Tapscott, Stephen J.

In: Molecular Cell, Vol. 14, No. 4, 21.05.2004, p. 465-477.

Research output: Contribution to journalArticle

Berkes, Charlotte A. ; Bergstrom, Donald A. ; Penn, Bennett ; Seaver, Karen J. ; Knoepfler, Paul S ; Tapscott, Stephen J. / Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potential. In: Molecular Cell. 2004 ; Vol. 14, No. 4. pp. 465-477.
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