Pax6 regulation of Math5 during mouse retinal neurogenesis

Amy N. Riesenberg, Tien T. Le, Minde I. Willardsen, David C. Blackburn, Monica L. Vetter, Nadean L Brown

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Activation of the bHLH factor Math5 (Atoh7) is an initiating event for mammalian retinal neurogenesis, as it is critically required for retinal ganglion cell formation. However, the cis-regulatory elements and transacting factors that control Math5 expression are largely unknown. Using a combination of transgenic mice and bioinformatics, we identified a phylogenetically conserved regulatory element that is required to activate Math5 transcription during early retinal neurogenesis. This element drives retinal expression in vivo, in a cross-species transgenic assay. Previously, Pax6 was shown to be necessary for the initiation of Math5 mRNA expression. We extend this finding by showing that the Math5 retinal enhancer also requires Pax6 for its activation, via Pax6 binding to a highly conserved binding site. In addition, our data reveal that other retinal factors are required for accurate regulation of Math5 by Pax6.

Original languageEnglish (US)
Pages (from-to)175-187
Number of pages13
JournalGenesis
Volume47
Issue number3
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Math5
  • Neurogenesis
  • Pax6
  • Retina
  • Transcriptional regulation

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Riesenberg, A. N., Le, T. T., Willardsen, M. I., Blackburn, D. C., Vetter, M. L., & Brown, N. L. (2009). Pax6 regulation of Math5 during mouse retinal neurogenesis. Genesis, 47(3), 175-187. https://doi.org/10.1002/dvg.20479