Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase b spindle elongation

Haifeng Wang, Ingrid Brust-Mascher, Gul Civelekoglu-Scholey, Jonathan M. Scholey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Anaphase B spindle elongation contributes to chromosome segregation during Drosophila melanogaster embryo mitosis. We propose that this process is driven by a kinesin-5-generated interpolar microtubule (MT; ipMT) sliding filament mechanism that engages when poleward flux is turned off. In this paper, we present evidence that anaphase B is induced by the minus end-stabilizing protein Patronin, which antagonizes the kinesin-13 depolymerase KLP10A at spindle poles, thereby switching offthe depolymerization of the minus ends of outwardly sliding ipMTs to suppress flux. Although intact cortices, kinetochore MTs, and midzone augmentation are dispensable, this Patronin-based change in ipMT minus-enddynamics is sufficient to induce the elongation of spindles capable of separating chromosomes.

Original languageEnglish (US)
Pages (from-to)35-46
Number of pages12
JournalJournal of Cell Biology
Volume203
Issue number1
DOIs
StatePublished - 2013

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Kinesin
Anaphase
Spindle Poles
Kinetochores
Chromosome Segregation
Drosophila melanogaster
Mitosis
Microtubules
Embryonic Structures
Chromosomes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase b spindle elongation. / Wang, Haifeng; Brust-Mascher, Ingrid; Civelekoglu-Scholey, Gul; Scholey, Jonathan M.

In: Journal of Cell Biology, Vol. 203, No. 1, 2013, p. 35-46.

Research output: Contribution to journalArticle

Wang, Haifeng ; Brust-Mascher, Ingrid ; Civelekoglu-Scholey, Gul ; Scholey, Jonathan M. / Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase b spindle elongation. In: Journal of Cell Biology. 2013 ; Vol. 203, No. 1. pp. 35-46.
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