Pathology of domoic acid toxicity in California sea lions (Zalophus californianus)

P. A. Silvagni, Linda J Lowenstine, T. Spraker, T. P. Lipscomb, F. M D Gulland

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Over 100 free-ranging adult California sea lions (Zalophus californianus) and one Northern fur seal (Callorhinus ursinus), predominantly adult females, were intoxicated by domoic acid (DA) during three harmful algal blooms between 1998 and 2000 in central and northern California coastal waters. The vector prey item was Northern anchovy (Engraulis mordax) and the primary DA-producing algal diatom was Psuedonitzschia australis. Postmortem examination revealed gross and histologic findings that were distinctive and aided in diagnosis. A total of 109 sea lions were examined, dying between 1 day and 10 months after admission to a marine mammal rehabilitation center. Persistent seizures with obtundation were the main clinical findings. Frequent gross findings in animals dying acutely consisted of piriform lobe malacia, myocardial pallor, bronchopneumonia, and complications related to pregnancy. Gross findings in animals dying months after intoxication included bilateral hippocampal atrophy. Histologic observations implicated limbic system seizure injury consistent with excitotoxin exposure. Peracutely, there was microvesicular hydropic degeneration within the neuropil of the hippocampus, amygdala, pyriform lobe, and other limbic structures. Acutely, there was ischemic neuronal necrosis, particularly apparent in the granular cells of the dentate gyrus and the pyramidal cells within the hippocampus cornu ammonis (CA) sectors CA4, CA3, and CA1. Dentate granular cell necrosis has not been reported in human or experimental animal DA toxicity and may be unique to sea lions. Chronically, there was gliosis, mild nonsuppurative inflammation, and loss of laminar organization in affected areas.

Original languageEnglish (US)
Pages (from-to)184-191
Number of pages8
JournalVeterinary Pathology
Volume42
Issue number2
DOIs
StatePublished - Mar 2005

Fingerprint

Sea Lions
domoic acid
Fur Seals
Otariidae
hippocampus
seizures
Pathology
toxicity
Hippocampus
necrosis
Callorhinus
bronchopneumonia
amygdala
Seizures
Necrosis
rehabilitation (people)
Bacillariophyceae
Harmful Algal Bloom
marine mammals
cells

Keywords

  • Amnesic shellfish poisoning
  • California sea lions
  • Domoic acid
  • Excitotoxin
  • Marine toxin
  • Neurotoxin
  • Red tide
  • Seizure
  • Zalophus californianus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Pathology of domoic acid toxicity in California sea lions (Zalophus californianus). / Silvagni, P. A.; Lowenstine, Linda J; Spraker, T.; Lipscomb, T. P.; Gulland, F. M D.

In: Veterinary Pathology, Vol. 42, No. 2, 03.2005, p. 184-191.

Research output: Contribution to journalArticle

Silvagni, P. A. ; Lowenstine, Linda J ; Spraker, T. ; Lipscomb, T. P. ; Gulland, F. M D. / Pathology of domoic acid toxicity in California sea lions (Zalophus californianus). In: Veterinary Pathology. 2005 ; Vol. 42, No. 2. pp. 184-191.
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