Pathology of blackleg in cattle in California, 1991-2015

Camila C. Abreu, Patricia C Blanchard, John M Adaska, Robert B. Moeller, Mark L Anderson, Mauricio A. Navarro, Santiago S Diab, Francisco A Uzal

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Abstract

Blackleg is an infectious disease of cattle and rarely other ruminants, produced by Clostridium chauvoei and characterized by necrotizing myositis. In most cases of blackleg, the large muscles of the pectoral and pelvic girdles are affected, with other skeletal muscles and the heart involved less frequently. We studied 29 blackleg cases selected from the archives of the California Animal Health and Food Safety Laboratory, 1991-2015. Immunohistochemistry was also evaluated to detect C. chauvoei in formalin-fixed, paraffin-embedded (FFPE) tissues of cattle. Nineteen animals had gross and/or microscopic lesions in both skeletal muscle and heart, 9 had lesions in the skeletal musculature alone, and 1 in the heart alone. Gross lesions in the skeletal musculature involved the following muscle groups: hindquarters ( n = 8), forequarters ( n = 5), neck ( n = 5), lumbar area ( n = 3), brisket ( n = 2), diaphragm ( n = 2), abdominal wall ( n = 1), thoracic wall ( n = 1), and tongue ( n = 1). Of the 20 animals that had lesions in the heart, 11 had pericarditis and myocarditis; 7 had pericarditis, myocarditis, and endocarditis; and 1 each had pericarditis and myocarditis. Immunohistochemistry was 100% sensitive to detect C. chauvoei in FFPE skeletal muscle and/or heart of cattle with blackleg. Simultaneous lesions in skeletal musculature and heart were relatively common in blackleg cases in California; the most affected skeletal muscles were those of the hindlimbs.

Original languageEnglish (US)
Pages (from-to)894-901
Number of pages8
JournalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume30
Issue number6
DOIs
StatePublished - Nov 1 2018

Fingerprint

blackleg (animal disease)
Clostridium chauvoei
lesions (animal)
heart
Pathology
pericarditis
myocarditis
Pericarditis
skeletal muscle
cattle
Myocarditis
Skeletal Muscle
formalin
Paraffin
alkanes
Formaldehyde
immunohistochemistry
Immunohistochemistry
Pectoralis Muscles
myositis

Keywords

  • Blackleg
  • cattle
  • Clostridium chauvoei
  • myocarditis
  • myositis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Pathology of blackleg in cattle in California, 1991-2015",
abstract = "Blackleg is an infectious disease of cattle and rarely other ruminants, produced by Clostridium chauvoei and characterized by necrotizing myositis. In most cases of blackleg, the large muscles of the pectoral and pelvic girdles are affected, with other skeletal muscles and the heart involved less frequently. We studied 29 blackleg cases selected from the archives of the California Animal Health and Food Safety Laboratory, 1991-2015. Immunohistochemistry was also evaluated to detect C. chauvoei in formalin-fixed, paraffin-embedded (FFPE) tissues of cattle. Nineteen animals had gross and/or microscopic lesions in both skeletal muscle and heart, 9 had lesions in the skeletal musculature alone, and 1 in the heart alone. Gross lesions in the skeletal musculature involved the following muscle groups: hindquarters ( n = 8), forequarters ( n = 5), neck ( n = 5), lumbar area ( n = 3), brisket ( n = 2), diaphragm ( n = 2), abdominal wall ( n = 1), thoracic wall ( n = 1), and tongue ( n = 1). Of the 20 animals that had lesions in the heart, 11 had pericarditis and myocarditis; 7 had pericarditis, myocarditis, and endocarditis; and 1 each had pericarditis and myocarditis. Immunohistochemistry was 100{\%} sensitive to detect C. chauvoei in FFPE skeletal muscle and/or heart of cattle with blackleg. Simultaneous lesions in skeletal musculature and heart were relatively common in blackleg cases in California; the most affected skeletal muscles were those of the hindlimbs.",
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author = "Abreu, {Camila C.} and Blanchard, {Patricia C} and Adaska, {John M} and Moeller, {Robert B.} and Anderson, {Mark L} and Navarro, {Mauricio A.} and Diab, {Santiago S} and Uzal, {Francisco A}",
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AU - Moeller, Robert B.

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AU - Navarro, Mauricio A.

AU - Diab, Santiago S

AU - Uzal, Francisco A

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