Pathological changes associated with white striping in broiler breast muscles

V. A. Kuttappan, H L Shivaprasad, D. P. Shaw, B. A. Valentine, B. M. Hargis, F. D. Clark, S. R. McKee, C. M. Owens

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

White striping is a condition in broiler chickens characterized grossly by the occurrence of white striations, seen parallel to the direction of muscle fibers, on broiler breast fillets and thighs. Based on visual evaluation of the intensity of white striping, breast fillets can be categorized into normal (NORM), moderate (MOD), and severe (SEV) categories. This study was undertaken to evaluate the details of changes in histology as well as proximate composition occurring in the fillets with respect to the 3 degrees of white striping. In experiment 1, Rep.resentative breast fillets for each degree of white striping (n = 20) were collected from 45-d-old broilers, approximately 2 h postmortem. From each fillet, 2 skeletal muscle samples were obtained and fixed in 10% neutral buffered formalin. To identify and differentiate the histological changes, slides were pRep.ared and stained using hematoxylin and eosin, Masson's Trichrome, and Oil Red O stains. In experiment 2, samples with 3 degrees of white striping were collected from 57-d-old birds for conducting proximate analysis. Major histopathological changes observed in the MOD and SEV samples consisted of loss of cross striations, variability in fiber size, floccular/ vacuolar degeneration and lysis of fibers, mild mineralization, occasional regeneration (nuclear rowing and multinucleated cells), mononuclear cell infiltration, lipidosis, and interstitial inflammation and fibrosis. Microscopic lesions were visually scored for degeneration and necrosis, fibrosis, and lipidosis. The scale used to score the samples ranged from 0 (normal) to 3 (severe). There was an increase (P < 0.05) in mean scores for degenerative or necrotic lesions, fibrosis, and lipidosis as the degree of white striping increased from NORM to SEV. The results from the histopathological study were supported by the findings from proximate analysis confirming that the fat and protein contents of muscle increased (P < 0.05) and decreased (P < 0.05), respectively, as the degree of white striping increased. In conclusion, the histopathological changes occurring in white striping indicate a degenerative myopathy that could be associated with increased growth rate in birds.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalPoultry Science
Volume92
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

breast muscle
fillets
broiler chickens
proximate composition
fibrosis
breasts
lesions (animal)
dietary fiber
sampling
muscular diseases
birds
thighs
formalin
muscle fibers
histology
skeletal muscle
mineralization
necrosis
inflammation
lipid content

Keywords

  • Broiler breast fillet
  • Myopathy
  • Pathology
  • Proximate analysis
  • White striping

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Kuttappan, V. A., Shivaprasad, H. L., Shaw, D. P., Valentine, B. A., Hargis, B. M., Clark, F. D., ... Owens, C. M. (2013). Pathological changes associated with white striping in broiler breast muscles. Poultry Science, 92(2), 331-338. https://doi.org/10.3382/ps.2012-02646

Pathological changes associated with white striping in broiler breast muscles. / Kuttappan, V. A.; Shivaprasad, H L; Shaw, D. P.; Valentine, B. A.; Hargis, B. M.; Clark, F. D.; McKee, S. R.; Owens, C. M.

In: Poultry Science, Vol. 92, No. 2, 02.2013, p. 331-338.

Research output: Contribution to journalArticle

Kuttappan, VA, Shivaprasad, HL, Shaw, DP, Valentine, BA, Hargis, BM, Clark, FD, McKee, SR & Owens, CM 2013, 'Pathological changes associated with white striping in broiler breast muscles', Poultry Science, vol. 92, no. 2, pp. 331-338. https://doi.org/10.3382/ps.2012-02646
Kuttappan, V. A. ; Shivaprasad, H L ; Shaw, D. P. ; Valentine, B. A. ; Hargis, B. M. ; Clark, F. D. ; McKee, S. R. ; Owens, C. M. / Pathological changes associated with white striping in broiler breast muscles. In: Poultry Science. 2013 ; Vol. 92, No. 2. pp. 331-338.
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