Pathological alterations of the cerebral microvasculature in Alzheimer's disease and related dementing disorders

L. Buée, P. R. Hof, C. Bouras, A. Delacourte, D. P. Perl, John Morrison, H. M. Fillit

Research output: Contribution to journalArticle

231 Citations (Scopus)

Abstract

Alterations of the cerebral microvasculature have been reported in aging and in neurodegenerative disorders such as Alzheimer's disease. However, the exact role of microvascular alterations in the pathogenesis of neurodegeneration remains unknown. In the present report, the cerebral cortex microvasculature was studied by immunohistochemistry using a monoclonal antibody against vascular heparan sulfate proteoglycan protein core in normal aging controls, Alzheimer's disease, Down syndrome, Guam amyotrophic lateral sclerosis/parkinsonian dementia complex, Pick's disease and dementia pugilistica. In all dementing illnesses, increased microvascular pathology was evident compared to normal controls. Decreased microvascular density and numerous atrophic vessels were the primary abnormalities observed in all dementing disorders. These microvascular abnormalities demonstrated regional and laminar selectivity, and were primarly found in layers III and V of frontal and temporal cortex. Quantitative analysis employing computer-assisted microscopy demonstrated that the decrease in microvascular density in Alzheimer's disease was statistically significant compared to age-matched controls. In addition, extracellular heparan sulfate proteoglycan deposits were observed which colocalized with thioflavine S-positive senile plaques in Alzheimer's disease, Down syndrome and selected Guam dementia cases. In some cases, heparan sulfate proteoglycan was seen in senile plaques that appeared to be diffuse or primitive plaques that stained weakly with thioflavine. Heparan sulfate proteoglycan-containing neurons were also observed in Alzheimer's disease, as well as in Down syndrome and Guam cases. Glial staining for heparan sulfate proteoglycan was never observed. Our data support previous observations that microvascular pathology is found in aging and in Alzheimer's disease. The changes in Alzheimer's disease exceed those found in normal aging controls. We also found microvascular pathology in all other dementing disorders studied. Our studies further demonstrated that the microvascular pathology displays regional and laminar patterns which parallel patterns of neuronal loss. Finally, we also found that heparan sulfate proteoglycan is present in senile plaques and neurons not only as previously reported in Alzheimer's disease, but also in Down syndrome and Guam cases. Heparan sulfate proteoglycan in senile plaques may be derived from either the degenerating microvasculature or from degenerating neurons. Further studies are necessary to determine the role of microvascular disease in the progression of Alzheimer's disease and other dementing disorders.

Original languageEnglish (US)
Pages (from-to)469-480
Number of pages12
JournalActa Neuropathologica
Volume87
Issue number5
DOIs
StatePublished - May 1 1994
Externally publishedYes

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Microvessels
Heparan Sulfate Proteoglycans
Alzheimer Disease
Guam
Amyloid Plaques
Down Syndrome
Pathology
Dementia
Neurons
Syndecan-2
Pick Disease of the Brain
Amyotrophic Lateral Sclerosis
Frontal Lobe
Temporal Lobe
Neuroglia
Neurodegenerative Diseases
Cerebral Cortex
Blood Vessels
Disease Progression
Microscopy

Keywords

  • Alzheimer's disease Cerebral cortex
  • Dementia Heparan sulfate proteoglycan
  • Microvasculature

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Pathological alterations of the cerebral microvasculature in Alzheimer's disease and related dementing disorders. / Buée, L.; Hof, P. R.; Bouras, C.; Delacourte, A.; Perl, D. P.; Morrison, John; Fillit, H. M.

In: Acta Neuropathologica, Vol. 87, No. 5, 01.05.1994, p. 469-480.

Research output: Contribution to journalArticle

Buée, L. ; Hof, P. R. ; Bouras, C. ; Delacourte, A. ; Perl, D. P. ; Morrison, John ; Fillit, H. M. / Pathological alterations of the cerebral microvasculature in Alzheimer's disease and related dementing disorders. In: Acta Neuropathologica. 1994 ; Vol. 87, No. 5. pp. 469-480.
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