Pathologic and Prognostic Characteristics of Splenomegaly in Dogs Due to Fibrohistiocytic Nodules

98 Cases

W. L. Spangler, Philip H Kass

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Ninety-eight canine splenectomy specimens consisting of combined nodular lymphoid and fibrohistiocytic cell proliferation were evaluated for seven light microscopic characteristics. Electron microscopic features in eight primary and two metastatic nodules (liver) were also evaluated. Nodular fibrohistiocytic proliferation in the canine spleen is characterized by a mixed population of histiocytoid and/or spindle cells in varying proportions intermixed with hematopoietic elements, plasma cells, and/or lymphocytes. These nodules seem to form a continuum between splenic lymphoid nodular hyperplasia and malignant splenic stromal neoplasms (malignant fibrous histiocytoma). Immunohistochemical methods used on 32/98 specimens showed uniform and strong positive staining among fibrohistiocytic cells for vimentin and desmin; S100 protein was similarly stained in general abundance. Individual cells strongly stained with smooth muscle actin were sparse but widely distributed. Proliferating cell nuclear antigen was not useful in the subjective differentiation of nodules taken from dogs that died of spleen-related causes and those surviving 12 months following splenectomy. A spectrum of cell types were observed by electron microscopy within each nodule. Fibroblasts, macrophages, intermediate fibrohistiocytic types, and several forms of splenic reticular cells were present. There were no consistent alterations in hematology or serum chemistry profiles of these dogs to provide useful diagnostic/prognostic information. Among the 93/98 dogs with complete (12 month) follow-up information, 48% (45/93) were alive and 52% (48/93) were dead. Dogs that died or were euthanatized during the follow-up period had a median survival of 5 and 5.5 months, respectively (range 0-15 months). Forty-four percent (21/48) died from causes linked to their splenic disease, and 35% (17/48) died from competing causes. The cause of death in 21% (10/48) was unknown. Lymphoid : fibrohistiocytic proportion and mitotic index in the nodules were anatomic features most predictive of postsplenectomy mortality. A higher proportion of lymphoid to fibrohistiocytic type cells was associated with increased long-term survival, whereas lower lymphoid : fibrohistiocytic proportions and higher mitotic index indicated a probability of higher short-term mortality.

Original languageEnglish (US)
Pages (from-to)488-498
Number of pages11
JournalVeterinary Pathology
Volume35
Issue number6
StatePublished - Nov 1998

Fingerprint

splenomegaly
Splenomegaly
Dogs
dogs
Mitotic Index
Splenectomy
cells
Canidae
Splenic Neoplasms
splenic diseases
Splenic Diseases
spleen
Spleen
Lymphocytes
Malignant Fibrous Histiocytoma
desmin
proliferating cell nuclear antigen
blood chemistry
Desmin
S100 Proteins

Keywords

  • Dogs
  • Electron microscopy
  • Fibrohistiocytic nodules
  • Immunohistochemistry
  • Malignant fibrous histiocytoma
  • Mitotic index
  • Patient survival
  • Spleen

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Pathologic and Prognostic Characteristics of Splenomegaly in Dogs Due to Fibrohistiocytic Nodules : 98 Cases. / Spangler, W. L.; Kass, Philip H.

In: Veterinary Pathology, Vol. 35, No. 6, 11.1998, p. 488-498.

Research output: Contribution to journalArticle

@article{e805f48700f948369add45b81d45bfb6,
title = "Pathologic and Prognostic Characteristics of Splenomegaly in Dogs Due to Fibrohistiocytic Nodules: 98 Cases",
abstract = "Ninety-eight canine splenectomy specimens consisting of combined nodular lymphoid and fibrohistiocytic cell proliferation were evaluated for seven light microscopic characteristics. Electron microscopic features in eight primary and two metastatic nodules (liver) were also evaluated. Nodular fibrohistiocytic proliferation in the canine spleen is characterized by a mixed population of histiocytoid and/or spindle cells in varying proportions intermixed with hematopoietic elements, plasma cells, and/or lymphocytes. These nodules seem to form a continuum between splenic lymphoid nodular hyperplasia and malignant splenic stromal neoplasms (malignant fibrous histiocytoma). Immunohistochemical methods used on 32/98 specimens showed uniform and strong positive staining among fibrohistiocytic cells for vimentin and desmin; S100 protein was similarly stained in general abundance. Individual cells strongly stained with smooth muscle actin were sparse but widely distributed. Proliferating cell nuclear antigen was not useful in the subjective differentiation of nodules taken from dogs that died of spleen-related causes and those surviving 12 months following splenectomy. A spectrum of cell types were observed by electron microscopy within each nodule. Fibroblasts, macrophages, intermediate fibrohistiocytic types, and several forms of splenic reticular cells were present. There were no consistent alterations in hematology or serum chemistry profiles of these dogs to provide useful diagnostic/prognostic information. Among the 93/98 dogs with complete (12 month) follow-up information, 48{\%} (45/93) were alive and 52{\%} (48/93) were dead. Dogs that died or were euthanatized during the follow-up period had a median survival of 5 and 5.5 months, respectively (range 0-15 months). Forty-four percent (21/48) died from causes linked to their splenic disease, and 35{\%} (17/48) died from competing causes. The cause of death in 21{\%} (10/48) was unknown. Lymphoid : fibrohistiocytic proportion and mitotic index in the nodules were anatomic features most predictive of postsplenectomy mortality. A higher proportion of lymphoid to fibrohistiocytic type cells was associated with increased long-term survival, whereas lower lymphoid : fibrohistiocytic proportions and higher mitotic index indicated a probability of higher short-term mortality.",
keywords = "Dogs, Electron microscopy, Fibrohistiocytic nodules, Immunohistochemistry, Malignant fibrous histiocytoma, Mitotic index, Patient survival, Spleen",
author = "Spangler, {W. L.} and Kass, {Philip H}",
year = "1998",
month = "11",
language = "English (US)",
volume = "35",
pages = "488--498",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "6",

}

TY - JOUR

T1 - Pathologic and Prognostic Characteristics of Splenomegaly in Dogs Due to Fibrohistiocytic Nodules

T2 - 98 Cases

AU - Spangler, W. L.

AU - Kass, Philip H

PY - 1998/11

Y1 - 1998/11

N2 - Ninety-eight canine splenectomy specimens consisting of combined nodular lymphoid and fibrohistiocytic cell proliferation were evaluated for seven light microscopic characteristics. Electron microscopic features in eight primary and two metastatic nodules (liver) were also evaluated. Nodular fibrohistiocytic proliferation in the canine spleen is characterized by a mixed population of histiocytoid and/or spindle cells in varying proportions intermixed with hematopoietic elements, plasma cells, and/or lymphocytes. These nodules seem to form a continuum between splenic lymphoid nodular hyperplasia and malignant splenic stromal neoplasms (malignant fibrous histiocytoma). Immunohistochemical methods used on 32/98 specimens showed uniform and strong positive staining among fibrohistiocytic cells for vimentin and desmin; S100 protein was similarly stained in general abundance. Individual cells strongly stained with smooth muscle actin were sparse but widely distributed. Proliferating cell nuclear antigen was not useful in the subjective differentiation of nodules taken from dogs that died of spleen-related causes and those surviving 12 months following splenectomy. A spectrum of cell types were observed by electron microscopy within each nodule. Fibroblasts, macrophages, intermediate fibrohistiocytic types, and several forms of splenic reticular cells were present. There were no consistent alterations in hematology or serum chemistry profiles of these dogs to provide useful diagnostic/prognostic information. Among the 93/98 dogs with complete (12 month) follow-up information, 48% (45/93) were alive and 52% (48/93) were dead. Dogs that died or were euthanatized during the follow-up period had a median survival of 5 and 5.5 months, respectively (range 0-15 months). Forty-four percent (21/48) died from causes linked to their splenic disease, and 35% (17/48) died from competing causes. The cause of death in 21% (10/48) was unknown. Lymphoid : fibrohistiocytic proportion and mitotic index in the nodules were anatomic features most predictive of postsplenectomy mortality. A higher proportion of lymphoid to fibrohistiocytic type cells was associated with increased long-term survival, whereas lower lymphoid : fibrohistiocytic proportions and higher mitotic index indicated a probability of higher short-term mortality.

AB - Ninety-eight canine splenectomy specimens consisting of combined nodular lymphoid and fibrohistiocytic cell proliferation were evaluated for seven light microscopic characteristics. Electron microscopic features in eight primary and two metastatic nodules (liver) were also evaluated. Nodular fibrohistiocytic proliferation in the canine spleen is characterized by a mixed population of histiocytoid and/or spindle cells in varying proportions intermixed with hematopoietic elements, plasma cells, and/or lymphocytes. These nodules seem to form a continuum between splenic lymphoid nodular hyperplasia and malignant splenic stromal neoplasms (malignant fibrous histiocytoma). Immunohistochemical methods used on 32/98 specimens showed uniform and strong positive staining among fibrohistiocytic cells for vimentin and desmin; S100 protein was similarly stained in general abundance. Individual cells strongly stained with smooth muscle actin were sparse but widely distributed. Proliferating cell nuclear antigen was not useful in the subjective differentiation of nodules taken from dogs that died of spleen-related causes and those surviving 12 months following splenectomy. A spectrum of cell types were observed by electron microscopy within each nodule. Fibroblasts, macrophages, intermediate fibrohistiocytic types, and several forms of splenic reticular cells were present. There were no consistent alterations in hematology or serum chemistry profiles of these dogs to provide useful diagnostic/prognostic information. Among the 93/98 dogs with complete (12 month) follow-up information, 48% (45/93) were alive and 52% (48/93) were dead. Dogs that died or were euthanatized during the follow-up period had a median survival of 5 and 5.5 months, respectively (range 0-15 months). Forty-four percent (21/48) died from causes linked to their splenic disease, and 35% (17/48) died from competing causes. The cause of death in 21% (10/48) was unknown. Lymphoid : fibrohistiocytic proportion and mitotic index in the nodules were anatomic features most predictive of postsplenectomy mortality. A higher proportion of lymphoid to fibrohistiocytic type cells was associated with increased long-term survival, whereas lower lymphoid : fibrohistiocytic proportions and higher mitotic index indicated a probability of higher short-term mortality.

KW - Dogs

KW - Electron microscopy

KW - Fibrohistiocytic nodules

KW - Immunohistochemistry

KW - Malignant fibrous histiocytoma

KW - Mitotic index

KW - Patient survival

KW - Spleen

UR - http://www.scopus.com/inward/record.url?scp=0032201369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032201369&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 488

EP - 498

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 6

ER -