Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks

A. S. Dhillon, B. Alisantosa, H L Shivaprasad, O. Jack, D. Schaberg, D. Bandli

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Four hundred fifty day-old Hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. Six groups of chicks received 0.5 ml of broth culture containing 5 x 106 colony-forming units (CFU) of Salmonella enteritidis (SE) phage types (PTs) 4, 8, and 23 by crop gavage. Similarly, six other groups received 0.5 ml containing 5 x 108 CFU of SE. One group was inoculated with 0.5 ml containing 5 x 106 CFU of Salmonella pullorum, and another group received 0.5 ml containing 5 x 108 CFU of S. pullorum. A group of 30 chicks were kept as uninoculated controls. Chicks were observed daily for clinical signs and mortality. All birds were weighed at 7, 14, and 21 days postinoculation 21 (DPI). Four chicks were randomly selected from each treatment group, euthanatized, and necropsied at 7 and 14 DPI. Gross lesions were recorded and selected tissues were collected for histopathology. The higher rates of illness and mortality were observed in chicks inoculated with 5 x 106 and 5 x 108 CFU of S. pullorum, followed by SE PT4 of human origin and SE PT4 of chicken origin. Moderate to high mortality was observed in chicks inoculated with the higher dose of SE isolates that belonged to PT8 and one SE of PT23. Variable mortality was evident in groups inoculated with the lower dose of salmonella. The most consistent gross and histopathologic changes, including fibrinous pericarditis and perihepatitis, were seen in the dead birds from various treatment groups. The lower mean body weights were present in all treatment groups compared with uninoculated controls. No illness or mortality was observed in uninoculated control groups.

Original languageEnglish (US)
Pages (from-to)506-515
Number of pages10
JournalAvian Diseases
Volume43
Issue number3
StatePublished - Jul 1999

Fingerprint

Salmonella Phages
Salmonella enteritidis
Salmonella Enteritidis
bacteriophages
Virulence
pathogenicity
broiler chickens
chicks
Stem Cells
Salmonella Pullorum
Mortality
Salmonella
Birds
Hubbard (chicken breed)
Pericarditis
pericarditis
birds
dosage
Chickens
lesions (animal)

Keywords

  • Broiler
  • Infection
  • Salmonella enteritidis

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Dhillon, A. S., Alisantosa, B., Shivaprasad, H. L., Jack, O., Schaberg, D., & Bandli, D. (1999). Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks. Avian Diseases, 43(3), 506-515.

Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks. / Dhillon, A. S.; Alisantosa, B.; Shivaprasad, H L; Jack, O.; Schaberg, D.; Bandli, D.

In: Avian Diseases, Vol. 43, No. 3, 07.1999, p. 506-515.

Research output: Contribution to journalArticle

Dhillon, AS, Alisantosa, B, Shivaprasad, HL, Jack, O, Schaberg, D & Bandli, D 1999, 'Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks', Avian Diseases, vol. 43, no. 3, pp. 506-515.
Dhillon AS, Alisantosa B, Shivaprasad HL, Jack O, Schaberg D, Bandli D. Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks. Avian Diseases. 1999 Jul;43(3):506-515.
Dhillon, A. S. ; Alisantosa, B. ; Shivaprasad, H L ; Jack, O. ; Schaberg, D. ; Bandli, D. / Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks. In: Avian Diseases. 1999 ; Vol. 43, No. 3. pp. 506-515.
@article{90522a6506b34d1ab0a83deada31c93f,
title = "Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks",
abstract = "Four hundred fifty day-old Hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. Six groups of chicks received 0.5 ml of broth culture containing 5 x 106 colony-forming units (CFU) of Salmonella enteritidis (SE) phage types (PTs) 4, 8, and 23 by crop gavage. Similarly, six other groups received 0.5 ml containing 5 x 108 CFU of SE. One group was inoculated with 0.5 ml containing 5 x 106 CFU of Salmonella pullorum, and another group received 0.5 ml containing 5 x 108 CFU of S. pullorum. A group of 30 chicks were kept as uninoculated controls. Chicks were observed daily for clinical signs and mortality. All birds were weighed at 7, 14, and 21 days postinoculation 21 (DPI). Four chicks were randomly selected from each treatment group, euthanatized, and necropsied at 7 and 14 DPI. Gross lesions were recorded and selected tissues were collected for histopathology. The higher rates of illness and mortality were observed in chicks inoculated with 5 x 106 and 5 x 108 CFU of S. pullorum, followed by SE PT4 of human origin and SE PT4 of chicken origin. Moderate to high mortality was observed in chicks inoculated with the higher dose of SE isolates that belonged to PT8 and one SE of PT23. Variable mortality was evident in groups inoculated with the lower dose of salmonella. The most consistent gross and histopathologic changes, including fibrinous pericarditis and perihepatitis, were seen in the dead birds from various treatment groups. The lower mean body weights were present in all treatment groups compared with uninoculated controls. No illness or mortality was observed in uninoculated control groups.",
keywords = "Broiler, Infection, Salmonella enteritidis",
author = "Dhillon, {A. S.} and B. Alisantosa and Shivaprasad, {H L} and O. Jack and D. Schaberg and D. Bandli",
year = "1999",
month = "7",
language = "English (US)",
volume = "43",
pages = "506--515",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "3",

}

TY - JOUR

T1 - Pathogenicity of Salmonella enteritidis phage types 4, 8, and 23 in broiler chicks

AU - Dhillon, A. S.

AU - Alisantosa, B.

AU - Shivaprasad, H L

AU - Jack, O.

AU - Schaberg, D.

AU - Bandli, D.

PY - 1999/7

Y1 - 1999/7

N2 - Four hundred fifty day-old Hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. Six groups of chicks received 0.5 ml of broth culture containing 5 x 106 colony-forming units (CFU) of Salmonella enteritidis (SE) phage types (PTs) 4, 8, and 23 by crop gavage. Similarly, six other groups received 0.5 ml containing 5 x 108 CFU of SE. One group was inoculated with 0.5 ml containing 5 x 106 CFU of Salmonella pullorum, and another group received 0.5 ml containing 5 x 108 CFU of S. pullorum. A group of 30 chicks were kept as uninoculated controls. Chicks were observed daily for clinical signs and mortality. All birds were weighed at 7, 14, and 21 days postinoculation 21 (DPI). Four chicks were randomly selected from each treatment group, euthanatized, and necropsied at 7 and 14 DPI. Gross lesions were recorded and selected tissues were collected for histopathology. The higher rates of illness and mortality were observed in chicks inoculated with 5 x 106 and 5 x 108 CFU of S. pullorum, followed by SE PT4 of human origin and SE PT4 of chicken origin. Moderate to high mortality was observed in chicks inoculated with the higher dose of SE isolates that belonged to PT8 and one SE of PT23. Variable mortality was evident in groups inoculated with the lower dose of salmonella. The most consistent gross and histopathologic changes, including fibrinous pericarditis and perihepatitis, were seen in the dead birds from various treatment groups. The lower mean body weights were present in all treatment groups compared with uninoculated controls. No illness or mortality was observed in uninoculated control groups.

AB - Four hundred fifty day-old Hubbard broiler chicks were subdivided into 15 groups of 30 chicks each. Six groups of chicks received 0.5 ml of broth culture containing 5 x 106 colony-forming units (CFU) of Salmonella enteritidis (SE) phage types (PTs) 4, 8, and 23 by crop gavage. Similarly, six other groups received 0.5 ml containing 5 x 108 CFU of SE. One group was inoculated with 0.5 ml containing 5 x 106 CFU of Salmonella pullorum, and another group received 0.5 ml containing 5 x 108 CFU of S. pullorum. A group of 30 chicks were kept as uninoculated controls. Chicks were observed daily for clinical signs and mortality. All birds were weighed at 7, 14, and 21 days postinoculation 21 (DPI). Four chicks were randomly selected from each treatment group, euthanatized, and necropsied at 7 and 14 DPI. Gross lesions were recorded and selected tissues were collected for histopathology. The higher rates of illness and mortality were observed in chicks inoculated with 5 x 106 and 5 x 108 CFU of S. pullorum, followed by SE PT4 of human origin and SE PT4 of chicken origin. Moderate to high mortality was observed in chicks inoculated with the higher dose of SE isolates that belonged to PT8 and one SE of PT23. Variable mortality was evident in groups inoculated with the lower dose of salmonella. The most consistent gross and histopathologic changes, including fibrinous pericarditis and perihepatitis, were seen in the dead birds from various treatment groups. The lower mean body weights were present in all treatment groups compared with uninoculated controls. No illness or mortality was observed in uninoculated control groups.

KW - Broiler

KW - Infection

KW - Salmonella enteritidis

UR - http://www.scopus.com/inward/record.url?scp=0033167437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033167437&partnerID=8YFLogxK

M3 - Article

C2 - 10494420

AN - SCOPUS:0033167437

VL - 43

SP - 506

EP - 515

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 3

ER -