Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks

A. S. Dhillon, H L Shivaprasad, P. Roy, B. Alisantosa, D. Schaberg, D. Bandli, S. Johnson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two hundred sixty 1-d-old specific pathogen-free (SPF), Single Comb White Leghorn chicks were used in this study to determine pathology caused by Salmonella enteritidis (SE) isolated from a poultry environment. The chicks were subdivided into 10 equal groups of 26 chicks each. Eight groups of chicks were inoculated individually with 0.5 mL of brain heart infusion broth culture containing 1 × 106 cfu of SE phage type (PT) -8 (1, 2, 3), SE PT5 A (1, 2), or SE PT4 (Ch-env-CA, chicken-CA, and human) by crop gavage. One group of 26 chicks were inoculated with 1 × 106 cfu of Salmonella pullorum per bird by crop gavage. Another group of 26 chicks were kept as an uninoculated control group. All the chicks were observed daily for clinical signs and mortality. Salmonella was reisolated from different organs at 7, 14, 21, and 28 postinoculation (DPI). All of the chicks were weighed individually at each interval. Two chicks at random from each group were euthanised and necropsied at each DPI for gross pathology. Selected tissues were examined for histopathological changes at 7 and 14 DPI. Dead chicks were examined for gross and histopathological lesions. Mortality rates were 30.7, 15.3, and 7.6% in the groups inoculated with S. pullorum, SE PT5A, and SE PT4 (chicken-CA), respectively. No mortality or clinical sign were observed in other treatment groups or in uninoculated control groups. Cecal pouches were found to be the ideal organ for reisolation of Salmlonella at acute or chronic infection compared with other organs. Mean body weights were reduced to 1.8 to 12.6% in inoculated groups compared with the uninoculated control group. The consistent gross and hispathological lesions were of peritonitis, perihepatitis, yolk sac infection, and enteritis. Subclinical Salmlonella infection identified in this study resulted in reduced body weights of inoculated birds compared with uninoculated controls.

Original languageEnglish (US)
Pages (from-to)1323-1328
Number of pages6
JournalPoultry Science
Volume80
Issue number9
StatePublished - Sep 2001

Fingerprint

Salmonella
pathogenicity
chicks
Salmonella Enteritidis
pathogens
Salmonella Pullorum
crop (digestive system)
lesions (animal)
infection
chickens
body weight
peritonitis
birds
yolk sac
enteritis
White Leghorn
pouches
bacteriophages
poultry
heart

Keywords

  • Histopathology
  • Infection
  • Pathology
  • Salmonella enteritidis
  • Salmonella isolation

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Dhillon, A. S., Shivaprasad, H. L., Roy, P., Alisantosa, B., Schaberg, D., Bandli, D., & Johnson, S. (2001). Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks. Poultry Science, 80(9), 1323-1328.

Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks. / Dhillon, A. S.; Shivaprasad, H L; Roy, P.; Alisantosa, B.; Schaberg, D.; Bandli, D.; Johnson, S.

In: Poultry Science, Vol. 80, No. 9, 09.2001, p. 1323-1328.

Research output: Contribution to journalArticle

Dhillon, AS, Shivaprasad, HL, Roy, P, Alisantosa, B, Schaberg, D, Bandli, D & Johnson, S 2001, 'Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks', Poultry Science, vol. 80, no. 9, pp. 1323-1328.
Dhillon AS, Shivaprasad HL, Roy P, Alisantosa B, Schaberg D, Bandli D et al. Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks. Poultry Science. 2001 Sep;80(9):1323-1328.
Dhillon, A. S. ; Shivaprasad, H L ; Roy, P. ; Alisantosa, B. ; Schaberg, D. ; Bandli, D. ; Johnson, S. / Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks. In: Poultry Science. 2001 ; Vol. 80, No. 9. pp. 1323-1328.
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