Abstract
Two hundred sixty 1-d-old specific pathogen-free (SPF), Single Comb White Leghorn chicks were used in this study to determine pathology caused by Salmonella enteritidis (SE) isolated from a poultry environment. The chicks were subdivided into 10 equal groups of 26 chicks each. Eight groups of chicks were inoculated individually with 0.5 mL of brain heart infusion broth culture containing 1 × 106 cfu of SE phage type (PT) -8 (1, 2, 3), SE PT5 A (1, 2), or SE PT4 (Ch-env-CA, chicken-CA, and human) by crop gavage. One group of 26 chicks were inoculated with 1 × 106 cfu of Salmonella pullorum per bird by crop gavage. Another group of 26 chicks were kept as an uninoculated control group. All the chicks were observed daily for clinical signs and mortality. Salmonella was reisolated from different organs at 7, 14, 21, and 28 postinoculation (DPI). All of the chicks were weighed individually at each interval. Two chicks at random from each group were euthanised and necropsied at each DPI for gross pathology. Selected tissues were examined for histopathological changes at 7 and 14 DPI. Dead chicks were examined for gross and histopathological lesions. Mortality rates were 30.7, 15.3, and 7.6% in the groups inoculated with S. pullorum, SE PT5A, and SE PT4 (chicken-CA), respectively. No mortality or clinical sign were observed in other treatment groups or in uninoculated control groups. Cecal pouches were found to be the ideal organ for reisolation of Salmlonella at acute or chronic infection compared with other organs. Mean body weights were reduced to 1.8 to 12.6% in inoculated groups compared with the uninoculated control group. The consistent gross and hispathological lesions were of peritonitis, perihepatitis, yolk sac infection, and enteritis. Subclinical Salmlonella infection identified in this study resulted in reduced body weights of inoculated birds compared with uninoculated controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1323-1328 |
| Number of pages | 6 |
| Journal | Poultry Science |
| Volume | 80 |
| Issue number | 9 |
| State | Published - Sep 2001 |
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Keywords
- Histopathology
- Infection
- Pathology
- Salmonella enteritidis
- Salmonella isolation
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks. / Dhillon, A. S.; Shivaprasad, H L; Roy, P.; Alisantosa, B.; Schaberg, D.; Bandli, D.; Johnson, S.
In: Poultry Science, Vol. 80, No. 9, 09.2001, p. 1323-1328.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pathogenicity of environmental origin salmonellas in specific pathogen-free chicks
AU - Dhillon, A. S.
AU - Shivaprasad, H L
AU - Roy, P.
AU - Alisantosa, B.
AU - Schaberg, D.
AU - Bandli, D.
AU - Johnson, S.
PY - 2001/9
Y1 - 2001/9
N2 - Two hundred sixty 1-d-old specific pathogen-free (SPF), Single Comb White Leghorn chicks were used in this study to determine pathology caused by Salmonella enteritidis (SE) isolated from a poultry environment. The chicks were subdivided into 10 equal groups of 26 chicks each. Eight groups of chicks were inoculated individually with 0.5 mL of brain heart infusion broth culture containing 1 × 106 cfu of SE phage type (PT) -8 (1, 2, 3), SE PT5 A (1, 2), or SE PT4 (Ch-env-CA, chicken-CA, and human) by crop gavage. One group of 26 chicks were inoculated with 1 × 106 cfu of Salmonella pullorum per bird by crop gavage. Another group of 26 chicks were kept as an uninoculated control group. All the chicks were observed daily for clinical signs and mortality. Salmonella was reisolated from different organs at 7, 14, 21, and 28 postinoculation (DPI). All of the chicks were weighed individually at each interval. Two chicks at random from each group were euthanised and necropsied at each DPI for gross pathology. Selected tissues were examined for histopathological changes at 7 and 14 DPI. Dead chicks were examined for gross and histopathological lesions. Mortality rates were 30.7, 15.3, and 7.6% in the groups inoculated with S. pullorum, SE PT5A, and SE PT4 (chicken-CA), respectively. No mortality or clinical sign were observed in other treatment groups or in uninoculated control groups. Cecal pouches were found to be the ideal organ for reisolation of Salmlonella at acute or chronic infection compared with other organs. Mean body weights were reduced to 1.8 to 12.6% in inoculated groups compared with the uninoculated control group. The consistent gross and hispathological lesions were of peritonitis, perihepatitis, yolk sac infection, and enteritis. Subclinical Salmlonella infection identified in this study resulted in reduced body weights of inoculated birds compared with uninoculated controls.
AB - Two hundred sixty 1-d-old specific pathogen-free (SPF), Single Comb White Leghorn chicks were used in this study to determine pathology caused by Salmonella enteritidis (SE) isolated from a poultry environment. The chicks were subdivided into 10 equal groups of 26 chicks each. Eight groups of chicks were inoculated individually with 0.5 mL of brain heart infusion broth culture containing 1 × 106 cfu of SE phage type (PT) -8 (1, 2, 3), SE PT5 A (1, 2), or SE PT4 (Ch-env-CA, chicken-CA, and human) by crop gavage. One group of 26 chicks were inoculated with 1 × 106 cfu of Salmonella pullorum per bird by crop gavage. Another group of 26 chicks were kept as an uninoculated control group. All the chicks were observed daily for clinical signs and mortality. Salmonella was reisolated from different organs at 7, 14, 21, and 28 postinoculation (DPI). All of the chicks were weighed individually at each interval. Two chicks at random from each group were euthanised and necropsied at each DPI for gross pathology. Selected tissues were examined for histopathological changes at 7 and 14 DPI. Dead chicks were examined for gross and histopathological lesions. Mortality rates were 30.7, 15.3, and 7.6% in the groups inoculated with S. pullorum, SE PT5A, and SE PT4 (chicken-CA), respectively. No mortality or clinical sign were observed in other treatment groups or in uninoculated control groups. Cecal pouches were found to be the ideal organ for reisolation of Salmlonella at acute or chronic infection compared with other organs. Mean body weights were reduced to 1.8 to 12.6% in inoculated groups compared with the uninoculated control group. The consistent gross and hispathological lesions were of peritonitis, perihepatitis, yolk sac infection, and enteritis. Subclinical Salmlonella infection identified in this study resulted in reduced body weights of inoculated birds compared with uninoculated controls.
KW - Histopathology
KW - Infection
KW - Pathology
KW - Salmonella enteritidis
KW - Salmonella isolation
UR - http://www.scopus.com/inward/record.url?scp=0035461489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035461489&partnerID=8YFLogxK
M3 - Article
C2 - 11558918
AN - SCOPUS:0035461489
VL - 80
SP - 1323
EP - 1328
JO - Poultry Science
JF - Poultry Science
SN - 0032-5791
IS - 9
ER -