Pathogenicity associated with coinfection with very virulent infectious bursal disease and Infectious bursal disease virus strains endemic in the United States

Simone Stoute, Daral J. Jackwood, Susan E. Sommer-Wagner, Beate Crossley, Peter R. Woolcock, Bruce R. Charlton

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The pathogenicity induced by co-challenge with the rB strain of very virulent Infectious bursal disease virus (vvIBDV) and IBDV pathotypes endemic in the United States was evaluated in specific pathogen-free chickens. Four- and 6-week-old birds were simultaneously challenged with a 105 50% egg infectious dose (EID50) of rB mixed with a 105 EID50 of one of the following viruses: standard classic (STC), subclinical variant (Del-E), subclinical variant (T1), or avirulent serotype 2 (OH). Each challenge group consisted of 5 chickens. The severity of disease was assessed by comparing the 5-day mortality rates, bursal lesions (mean bursal lesion scores), and mean bursal-to-body weight ratios in each of the challenged groups. A mortality of 100% (10/10 and 5/5) was observed in birds inoculated with only the vvIBDV (rB) strain at 4 weeks and 6 weeks of age, respectively. Although the sample sizes were low, a significant reduction in mortality and severity of disease, based on mean bursal lesion scores, was observed in groups co-challenged with rB and the less virulent pathotypes Del-E, T1, or OH at 4 weeks of age. Co-challenge with rB and the antigenically similar STC strain did not result in a significant decrease in mortality compared to challenge with the pathogenic rB strain at 4 weeks of age, but a significant reduction in the mean bursa lesion score was observed. At 6 weeks of age, a significant decrease in mortality and mean bursa lesion score was observed in the rB groups co-challenged with STC, Del-E, or T1 but not OH.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume25
Issue number3
DOIs
StatePublished - May 2013

Fingerprint

Infectious bursal disease virus
infectious bursal disease
Coinfection
mixed infection
lesions (animal)
Communicable Diseases
Virulence
pathogenicity
Mortality
pathotypes
disease severity
Birds
Chickens
chickens
Specific Pathogen-Free Organisms
birds
Sample Size
Ovum
serotypes
Body Weight

Keywords

  • Chickens
  • co-challenge
  • pathogenicity
  • very virulent Infectious bursal disease virus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Pathogenicity associated with coinfection with very virulent infectious bursal disease and Infectious bursal disease virus strains endemic in the United States. / Stoute, Simone; Jackwood, Daral J.; Sommer-Wagner, Susan E.; Crossley, Beate; Woolcock, Peter R.; Charlton, Bruce R.

In: Journal of Veterinary Diagnostic Investigation, Vol. 25, No. 3, 05.2013, p. 352-358.

Research output: Contribution to journalArticle

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abstract = "The pathogenicity induced by co-challenge with the rB strain of very virulent Infectious bursal disease virus (vvIBDV) and IBDV pathotypes endemic in the United States was evaluated in specific pathogen-free chickens. Four- and 6-week-old birds were simultaneously challenged with a 105 50{\%} egg infectious dose (EID50) of rB mixed with a 105 EID50 of one of the following viruses: standard classic (STC), subclinical variant (Del-E), subclinical variant (T1), or avirulent serotype 2 (OH). Each challenge group consisted of 5 chickens. The severity of disease was assessed by comparing the 5-day mortality rates, bursal lesions (mean bursal lesion scores), and mean bursal-to-body weight ratios in each of the challenged groups. A mortality of 100{\%} (10/10 and 5/5) was observed in birds inoculated with only the vvIBDV (rB) strain at 4 weeks and 6 weeks of age, respectively. Although the sample sizes were low, a significant reduction in mortality and severity of disease, based on mean bursal lesion scores, was observed in groups co-challenged with rB and the less virulent pathotypes Del-E, T1, or OH at 4 weeks of age. Co-challenge with rB and the antigenically similar STC strain did not result in a significant decrease in mortality compared to challenge with the pathogenic rB strain at 4 weeks of age, but a significant reduction in the mean bursa lesion score was observed. At 6 weeks of age, a significant decrease in mortality and mean bursa lesion score was observed in the rB groups co-challenged with STC, Del-E, or T1 but not OH.",
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