Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort

Jason I. Feinberg; Kelly M. Bakulski; Andrew E. Jaffe; Rakel Tryggvadottir; Shannon C. Brown; Lynn R. Goldman; Lisa A. Croen; Irva Hertz-Picciotto; Craig J. Newschaffer; M. Daniele Fallin; Andrew P. Feinberg

doi:10.1093/ije/dyv028

Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort

Jason I. Feinberg, Kelly M. Bakulski, Andrew E. Jaffe, Rakel Tryggvadottir, Shannon C. Brown, Lynn R. Goldman, Lisa A. Croen, Irva Hertz-Picciotto, Craig J. Newschaffer, M. Daniele Fallin, Andrew P. Feinberg

Public Health Sciences

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Background: Epigenetic mechanisms such as altered DNA methylation have been suggested to play a role in autism, beginning with the classical association of Prader-Willi syndrome, an imprinting disorder, with autistic features. Objectives: Here we tested for the relationship of paternal sperm DNA methylation with autism risk in offspring, examining an enriched-risk cohort of fathers of autistic children. Methods: We examined genome-wide DNA methylation (DNAm) in paternal semen biosamples obtained from an autism spectrum disorder (ASD) enriched-risk pregnancy cohort, the Early Autism Risk Longitudinal Investigation (EARLI) cohort, to estimate associations between sperm DNAm and prospective ASD development, using a 12-month ASD symptoms assessment, the Autism Observation Scale for Infants (AOSI). We analysed methylation data from 44 sperm samples run on the CHARM 3.0 array, which contains over 4 million probes (over 7 million CpG sites), including 30 samples also run on the Illumina Infinium HumanMethylation450 (450K) BeadChip platform (~485 000 CpG sites). We also examined associated regions in an independent sample of postmortem human brain ASD and control samples for which Illumina 450K DNA methylation data were available. Results: Using region-based statistical approaches, we identified 193 differentially methylated regions (DMRs) in paternal sperm with a family-wise empirical P-value [family-wise error rate (FWER)]

Original language	English (US)
Pages (from-to)	1199-1210
Number of pages	12
Journal	International Journal of Epidemiology
Volume	44
Issue number	4
DOIs	https://doi.org/10.1093/ije/dyv028
State	Published - Aug 1 2015

Keywords

Autism risk
DNA methylation
Epigenetics
Sperm

ASJC Scopus subject areas

Epidemiology

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10.1093/ije/dyv028

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Feinberg, J. I., Bakulski, K. M., Jaffe, A. E., Tryggvadottir, R., Brown, S. C., Goldman, L. R., Croen, L. A., Hertz-Picciotto, I., Newschaffer, C. J., Daniele Fallin, M., & Feinberg, A. P. (2015). Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort. International Journal of Epidemiology, 44(4), 1199-1210. https://doi.org/10.1093/ije/dyv028

Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort. / Feinberg, Jason I.; Bakulski, Kelly M.; Jaffe, Andrew E.; Tryggvadottir, Rakel; Brown, Shannon C.; Goldman, Lynn R.; Croen, Lisa A.; Hertz-Picciotto, Irva; Newschaffer, Craig J.; Daniele Fallin, M.; Feinberg, Andrew P.

In: International Journal of Epidemiology, Vol. 44, No. 4, 01.08.2015, p. 1199-1210.

Research output: Contribution to journal › Article › peer-review

Feinberg, JI, Bakulski, KM, Jaffe, AE, Tryggvadottir, R, Brown, SC, Goldman, LR, Croen, LA, Hertz-Picciotto, I, Newschaffer, CJ, Daniele Fallin, M & Feinberg, AP 2015, 'Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort', International Journal of Epidemiology, vol. 44, no. 4, pp. 1199-1210. https://doi.org/10.1093/ije/dyv028

Feinberg, Jason I. ; Bakulski, Kelly M. ; Jaffe, Andrew E. ; Tryggvadottir, Rakel ; Brown, Shannon C. ; Goldman, Lynn R. ; Croen, Lisa A. ; Hertz-Picciotto, Irva ; Newschaffer, Craig J. ; Daniele Fallin, M. ; Feinberg, Andrew P. / Paternal sperm DNA methylation associated with early signs of autism risk in an autism-enriched cohort. In: International Journal of Epidemiology. 2015 ; Vol. 44, No. 4. pp. 1199-1210.

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abstract = "Background: Epigenetic mechanisms such as altered DNA methylation have been suggested to play a role in autism, beginning with the classical association of Prader-Willi syndrome, an imprinting disorder, with autistic features. Objectives: Here we tested for the relationship of paternal sperm DNA methylation with autism risk in offspring, examining an enriched-risk cohort of fathers of autistic children. Methods: We examined genome-wide DNA methylation (DNAm) in paternal semen biosamples obtained from an autism spectrum disorder (ASD) enriched-risk pregnancy cohort, the Early Autism Risk Longitudinal Investigation (EARLI) cohort, to estimate associations between sperm DNAm and prospective ASD development, using a 12-month ASD symptoms assessment, the Autism Observation Scale for Infants (AOSI). We analysed methylation data from 44 sperm samples run on the CHARM 3.0 array, which contains over 4 million probes (over 7 million CpG sites), including 30 samples also run on the Illumina Infinium HumanMethylation450 (450K) BeadChip platform (~485 000 CpG sites). We also examined associated regions in an independent sample of postmortem human brain ASD and control samples for which Illumina 450K DNA methylation data were available. Results: Using region-based statistical approaches, we identified 193 differentially methylated regions (DMRs) in paternal sperm with a family-wise empirical P-value [family-wise error rate (FWER)] ",

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AU - Brown, Shannon C.

AU - Goldman, Lynn R.

AU - Croen, Lisa A.

AU - Hertz-Picciotto, Irva

AU - Newschaffer, Craig J.

AU - Daniele Fallin, M.

AU - Feinberg, Andrew P.

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