Patch-clamp fluorometry recording of conformational rearrangements of ion channels.

Jie Zheng, William N. Zagotta

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Conformational rearrangements are commonly observed in membrane proteins and constitute the molecular mechanisms through which they contribute to signal transduction. Information on structural changes in membrane proteins has mostly been inferred from functional studies. Recently, site-specific fluorescence recordings have made it possible to directly observe such molecular events in real time within the membrane environment. Here, we describe the patch-clamp fluorometry (PCF) technique that records simultaneously the local structural changes and the functional states of ion channels in isolated cell membranes. Combined with fluorescence resonance energy transfer (FRET), the technique should shed new light on ion channel activation, regulation, and interaction with other membrane proteins.

Original languageEnglish (US)
JournalScience's STKE : signal transduction knowledge environment
Volume2003
Issue number176
StatePublished - 2003
Externally publishedYes

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Fluorometry
Clamping devices
Ion Channels
Membrane Proteins
Signal transduction
Fluorescence Resonance Energy Transfer
Patch-Clamp Techniques
Cell membranes
Signal Transduction
Fluorescence
Chemical activation
Cell Membrane
Membranes

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Patch-clamp fluorometry recording of conformational rearrangements of ion channels. / Zheng, Jie; Zagotta, William N.

In: Science's STKE : signal transduction knowledge environment, Vol. 2003, No. 176, 2003.

Research output: Contribution to journalArticle

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