Passaged goat costal chondrocytes provide a feasible cell source for temporomandibular joint tissue engineering

Deirdre E J Anderson, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Costal cartilage is commonly harvested for various types of facial reconstructive surgery. The ability of costal chondrocytes (CCs) to produce relevant extracellular matrix, including glycosaminoglycans (GAGs) and collagens, makes them an appealing cell source for fibrocartilage engineering. In order to obtain enough cells for tissue engineering, however, cell expansion will likely be necessary. This study examined CCs at passages 0, 1, 3, and 5, as well as temporomandibular (TMJ) disc cells, in a scaffoldless tissue engineering approach. It was hypothesized that earlier passage constructs would have more cartilaginous proteins and less fibrocartilaginous proteins. TMJ disc constructs had over twice the collagen content of any other group, as well as the largest tensile properties; however, the substantial contraction of the constructs and limited cell numbers make it a non-feasible cell source for tissue engineering. In general, statistical differences in mechanical properties or collagen content of the various CC groups were not observed; however, significantly more GAG was produced in the passaged CCs than the primary CCs. More collagen type II was also observed in some of the passaged groups. These results suggest not only feasibility but potential superiority of passaged CCs over primary CCs, which may lead to functional engineered fibrocartilage.

Original languageEnglish (US)
Pages (from-to)1992-2001
Number of pages10
JournalAnnals of Biomedical Engineering
Volume36
Issue number12
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Tissue engineering
Collagen
Proteins
Cartilage
Tensile properties
Surgery
Mechanical properties
Temporomandibular Joint
Glycosaminoglycans

Keywords

  • Cartilage
  • Extracellular matrix
  • Fibrocartilage
  • Fibrochondrocytes
  • Mechanical properties

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Passaged goat costal chondrocytes provide a feasible cell source for temporomandibular joint tissue engineering. / Anderson, Deirdre E J; Athanasiou, Kyriacos A.

In: Annals of Biomedical Engineering, Vol. 36, No. 12, 12.2008, p. 1992-2001.

Research output: Contribution to journalArticle

Anderson, Deirdre E J ; Athanasiou, Kyriacos A. / Passaged goat costal chondrocytes provide a feasible cell source for temporomandibular joint tissue engineering. In: Annals of Biomedical Engineering. 2008 ; Vol. 36, No. 12. pp. 1992-2001.
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