Abstract
Costal cartilage is commonly harvested for various types of facial reconstructive surgery. The ability of costal chondrocytes (CCs) to produce relevant extracellular matrix, including glycosaminoglycans (GAGs) and collagens, makes them an appealing cell source for fibrocartilage engineering. In order to obtain enough cells for tissue engineering, however, cell expansion will likely be necessary. This study examined CCs at passages 0, 1, 3, and 5, as well as temporomandibular (TMJ) disc cells, in a scaffoldless tissue engineering approach. It was hypothesized that earlier passage constructs would have more cartilaginous proteins and less fibrocartilaginous proteins. TMJ disc constructs had over twice the collagen content of any other group, as well as the largest tensile properties; however, the substantial contraction of the constructs and limited cell numbers make it a non-feasible cell source for tissue engineering. In general, statistical differences in mechanical properties or collagen content of the various CC groups were not observed; however, significantly more GAG was produced in the passaged CCs than the primary CCs. More collagen type II was also observed in some of the passaged groups. These results suggest not only feasibility but potential superiority of passaged CCs over primary CCs, which may lead to functional engineered fibrocartilage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1992-2001 |
| Number of pages | 10 |
| Journal | Annals of Biomedical Engineering |
| Volume | 36 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2008 |
| Externally published | Yes |
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Keywords
- Cartilage
- Extracellular matrix
- Fibrocartilage
- Fibrochondrocytes
- Mechanical properties
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Passaged goat costal chondrocytes provide a feasible cell source for temporomandibular joint tissue engineering. / Anderson, Deirdre E J; Athanasiou, Kyriacos A.
In: Annals of Biomedical Engineering, Vol. 36, No. 12, 12.2008, p. 1992-2001.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Passaged goat costal chondrocytes provide a feasible cell source for temporomandibular joint tissue engineering
AU - Anderson, Deirdre E J
AU - Athanasiou, Kyriacos A.
PY - 2008/12
Y1 - 2008/12
N2 - Costal cartilage is commonly harvested for various types of facial reconstructive surgery. The ability of costal chondrocytes (CCs) to produce relevant extracellular matrix, including glycosaminoglycans (GAGs) and collagens, makes them an appealing cell source for fibrocartilage engineering. In order to obtain enough cells for tissue engineering, however, cell expansion will likely be necessary. This study examined CCs at passages 0, 1, 3, and 5, as well as temporomandibular (TMJ) disc cells, in a scaffoldless tissue engineering approach. It was hypothesized that earlier passage constructs would have more cartilaginous proteins and less fibrocartilaginous proteins. TMJ disc constructs had over twice the collagen content of any other group, as well as the largest tensile properties; however, the substantial contraction of the constructs and limited cell numbers make it a non-feasible cell source for tissue engineering. In general, statistical differences in mechanical properties or collagen content of the various CC groups were not observed; however, significantly more GAG was produced in the passaged CCs than the primary CCs. More collagen type II was also observed in some of the passaged groups. These results suggest not only feasibility but potential superiority of passaged CCs over primary CCs, which may lead to functional engineered fibrocartilage.
AB - Costal cartilage is commonly harvested for various types of facial reconstructive surgery. The ability of costal chondrocytes (CCs) to produce relevant extracellular matrix, including glycosaminoglycans (GAGs) and collagens, makes them an appealing cell source for fibrocartilage engineering. In order to obtain enough cells for tissue engineering, however, cell expansion will likely be necessary. This study examined CCs at passages 0, 1, 3, and 5, as well as temporomandibular (TMJ) disc cells, in a scaffoldless tissue engineering approach. It was hypothesized that earlier passage constructs would have more cartilaginous proteins and less fibrocartilaginous proteins. TMJ disc constructs had over twice the collagen content of any other group, as well as the largest tensile properties; however, the substantial contraction of the constructs and limited cell numbers make it a non-feasible cell source for tissue engineering. In general, statistical differences in mechanical properties or collagen content of the various CC groups were not observed; however, significantly more GAG was produced in the passaged CCs than the primary CCs. More collagen type II was also observed in some of the passaged groups. These results suggest not only feasibility but potential superiority of passaged CCs over primary CCs, which may lead to functional engineered fibrocartilage.
KW - Cartilage
KW - Extracellular matrix
KW - Fibrocartilage
KW - Fibrochondrocytes
KW - Mechanical properties
UR - http://www.scopus.com/inward/record.url?scp=56449131350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56449131350&partnerID=8YFLogxK
U2 - 10.1007/s10439-008-9572-2
DO - 10.1007/s10439-008-9572-2
M3 - Article
C2 - 18830818
AN - SCOPUS:56449131350
VL - 36
SP - 1992
EP - 2001
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
SN - 0090-6964
IS - 12
ER -