Particulate matter in cigarette smoke alters iron homeostasis to produce a biological effect

Andrew J. Ghio, Elizabeth D. Hilborn, Jacqueline G. Stonehuerner, Lisa A. Dailey, Jacqueline D. Carter, Judy H. Richards, Kay M. Crissman, Robert F. Foronjy, Dale L. Uyeminami, Kent E Pinkerton

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Rationale: Lung injury after cigarette smoking is related to particle retention. Iron accumulates with the deposition of these particles. Objectives: We tested the postulate that (1) injury after smoking correlates with exposure to the particulate fraction of cigarette smoke, (2) these particles alter iron homeostasis, triggering metal accumulation, and (3) this alteration in iron homeostasis affects oxidative stress and inflammation. Methods: Rats and human respiratory epithelial cells were exposed to cigarette smoke, filtered cigarette smoke, and cigarette smoke condensate (the particulate fraction of smoke), and indices of iron homeostasis, oxidative stress, and inflammatory injury were determined. Comparable measures were also evaluated in nonsmokers and smokers. Measurements and Main Results: After exposure of rats to cigarette smoke, increased lavage concentrations of iron and ferritin, serum ferritin levels, and nonheme iron concentrations in the lung and liver tissue all increased. Lavage ascorbate concentrations were decreased, supporting an oxidative stress. After filtering of the cigarette smoke to remove particles, most of these changes were reversed. Exposure of cultured respiratory epithelial cells to cigarette smoke condensate caused a similar accumulation of iron, metal-dependent oxidative stress, and increased IL-8 release. Lavage samples in healthy smokers and smoking patients with chronic obstructive pulmonary disease revealed elevated concentrations of both iron and ferritin relative to healthy nonsmokers. Lavage ascorbate decreased with cigarette smoking. Serum iron and ferritin levels among smokers were increased, supporting systemic accumulation of this metal after cigarette smoke exposure. Conclusions: We conclude that cigarette smoke particles alter iron homeostasis, both in the lung and systemically.

Original languageEnglish (US)
Pages (from-to)1130-1138
Number of pages9
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume178
Issue number11
DOIs
StatePublished - Dec 1 2008

Fingerprint

Particulate Matter
Smoke
Tobacco Products
Homeostasis
Iron
Therapeutic Irrigation
Ferritins
Oxidative Stress
Smoking
Metals
Epithelial Cells
Lung
Wounds and Injuries
Lung Injury
Serum
Interleukin-8
Chronic Obstructive Pulmonary Disease
Inflammation

Keywords

  • Chronic obstructive pulmonary disease
  • Ferritin
  • Oxidants
  • Smoking

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Particulate matter in cigarette smoke alters iron homeostasis to produce a biological effect. / Ghio, Andrew J.; Hilborn, Elizabeth D.; Stonehuerner, Jacqueline G.; Dailey, Lisa A.; Carter, Jacqueline D.; Richards, Judy H.; Crissman, Kay M.; Foronjy, Robert F.; Uyeminami, Dale L.; Pinkerton, Kent E.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 178, No. 11, 01.12.2008, p. 1130-1138.

Research output: Contribution to journalArticle

Ghio, AJ, Hilborn, ED, Stonehuerner, JG, Dailey, LA, Carter, JD, Richards, JH, Crissman, KM, Foronjy, RF, Uyeminami, DL & Pinkerton, KE 2008, 'Particulate matter in cigarette smoke alters iron homeostasis to produce a biological effect', American Journal of Respiratory and Critical Care Medicine, vol. 178, no. 11, pp. 1130-1138. https://doi.org/10.1164/rccm.200802-334OC
Ghio, Andrew J. ; Hilborn, Elizabeth D. ; Stonehuerner, Jacqueline G. ; Dailey, Lisa A. ; Carter, Jacqueline D. ; Richards, Judy H. ; Crissman, Kay M. ; Foronjy, Robert F. ; Uyeminami, Dale L. ; Pinkerton, Kent E. / Particulate matter in cigarette smoke alters iron homeostasis to produce a biological effect. In: American Journal of Respiratory and Critical Care Medicine. 2008 ; Vol. 178, No. 11. pp. 1130-1138.
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abstract = "Rationale: Lung injury after cigarette smoking is related to particle retention. Iron accumulates with the deposition of these particles. Objectives: We tested the postulate that (1) injury after smoking correlates with exposure to the particulate fraction of cigarette smoke, (2) these particles alter iron homeostasis, triggering metal accumulation, and (3) this alteration in iron homeostasis affects oxidative stress and inflammation. Methods: Rats and human respiratory epithelial cells were exposed to cigarette smoke, filtered cigarette smoke, and cigarette smoke condensate (the particulate fraction of smoke), and indices of iron homeostasis, oxidative stress, and inflammatory injury were determined. Comparable measures were also evaluated in nonsmokers and smokers. Measurements and Main Results: After exposure of rats to cigarette smoke, increased lavage concentrations of iron and ferritin, serum ferritin levels, and nonheme iron concentrations in the lung and liver tissue all increased. Lavage ascorbate concentrations were decreased, supporting an oxidative stress. After filtering of the cigarette smoke to remove particles, most of these changes were reversed. Exposure of cultured respiratory epithelial cells to cigarette smoke condensate caused a similar accumulation of iron, metal-dependent oxidative stress, and increased IL-8 release. Lavage samples in healthy smokers and smoking patients with chronic obstructive pulmonary disease revealed elevated concentrations of both iron and ferritin relative to healthy nonsmokers. Lavage ascorbate decreased with cigarette smoking. Serum iron and ferritin levels among smokers were increased, supporting systemic accumulation of this metal after cigarette smoke exposure. Conclusions: We conclude that cigarette smoke particles alter iron homeostasis, both in the lung and systemically.",
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AU - Carter, Jacqueline D.

AU - Richards, Judy H.

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N2 - Rationale: Lung injury after cigarette smoking is related to particle retention. Iron accumulates with the deposition of these particles. Objectives: We tested the postulate that (1) injury after smoking correlates with exposure to the particulate fraction of cigarette smoke, (2) these particles alter iron homeostasis, triggering metal accumulation, and (3) this alteration in iron homeostasis affects oxidative stress and inflammation. Methods: Rats and human respiratory epithelial cells were exposed to cigarette smoke, filtered cigarette smoke, and cigarette smoke condensate (the particulate fraction of smoke), and indices of iron homeostasis, oxidative stress, and inflammatory injury were determined. Comparable measures were also evaluated in nonsmokers and smokers. Measurements and Main Results: After exposure of rats to cigarette smoke, increased lavage concentrations of iron and ferritin, serum ferritin levels, and nonheme iron concentrations in the lung and liver tissue all increased. Lavage ascorbate concentrations were decreased, supporting an oxidative stress. After filtering of the cigarette smoke to remove particles, most of these changes were reversed. Exposure of cultured respiratory epithelial cells to cigarette smoke condensate caused a similar accumulation of iron, metal-dependent oxidative stress, and increased IL-8 release. Lavage samples in healthy smokers and smoking patients with chronic obstructive pulmonary disease revealed elevated concentrations of both iron and ferritin relative to healthy nonsmokers. Lavage ascorbate decreased with cigarette smoking. Serum iron and ferritin levels among smokers were increased, supporting systemic accumulation of this metal after cigarette smoke exposure. Conclusions: We conclude that cigarette smoke particles alter iron homeostasis, both in the lung and systemically.

AB - Rationale: Lung injury after cigarette smoking is related to particle retention. Iron accumulates with the deposition of these particles. Objectives: We tested the postulate that (1) injury after smoking correlates with exposure to the particulate fraction of cigarette smoke, (2) these particles alter iron homeostasis, triggering metal accumulation, and (3) this alteration in iron homeostasis affects oxidative stress and inflammation. Methods: Rats and human respiratory epithelial cells were exposed to cigarette smoke, filtered cigarette smoke, and cigarette smoke condensate (the particulate fraction of smoke), and indices of iron homeostasis, oxidative stress, and inflammatory injury were determined. Comparable measures were also evaluated in nonsmokers and smokers. Measurements and Main Results: After exposure of rats to cigarette smoke, increased lavage concentrations of iron and ferritin, serum ferritin levels, and nonheme iron concentrations in the lung and liver tissue all increased. Lavage ascorbate concentrations were decreased, supporting an oxidative stress. After filtering of the cigarette smoke to remove particles, most of these changes were reversed. Exposure of cultured respiratory epithelial cells to cigarette smoke condensate caused a similar accumulation of iron, metal-dependent oxidative stress, and increased IL-8 release. Lavage samples in healthy smokers and smoking patients with chronic obstructive pulmonary disease revealed elevated concentrations of both iron and ferritin relative to healthy nonsmokers. Lavage ascorbate decreased with cigarette smoking. Serum iron and ferritin levels among smokers were increased, supporting systemic accumulation of this metal after cigarette smoke exposure. Conclusions: We conclude that cigarette smoke particles alter iron homeostasis, both in the lung and systemically.

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