Partial purification of the aggregation pheromone nippolure from female Nippostrongylus brasiliensis (Nematoda)

Leon W. Bone, Bruce D. Hammock, Lyle K. Gaston, Susan K. Reed, H. H. Shorey

Research output: Contribution to journalArticle

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Abstract

Partial purification of a pheromone of the zooparasitic nematode Nippostrongylus brasiliensis was achieved. The polypeptide pheromone is degraded by Pronase while increased recovery is obtained after treatment with the protease inhibitors phenylmethylsulfonyl fluoride or l-1-tosylamide-2-phenylethyl chloromethyl ketone. The pheromone has a net negative charge, based on retention on DEAE cellulose and an approximate isoelectric point of 7.3. A combination of double treatments with phenylmethylsulfonyl fluoride and gel filtration enables storage up to nine days which yields biological activity similar to fresh, untreated chromatographic fractions.

Original languageEnglish (US)
Pages (from-to)297-308
Number of pages12
JournalJournal of Chemical Ecology
Volume6
Issue number2
DOIs
StatePublished - Mar 1980

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Keywords

  • Nematoda
  • nematode sex pheromones
  • pheromone characterization
  • pheromone purification

ASJC Scopus subject areas

  • Ecology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

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