Partial purification of the aggregation pheromone nippolure from female Nippostrongylus brasiliensis (Nematoda)

Leon W. Bone; Bruce D. Hammock; Lyle K. Gaston; Susan K. Reed; H. H. Shorey

doi:10.1007/BF01402909

Partial purification of the aggregation pheromone nippolure from female Nippostrongylus brasiliensis (Nematoda)

Leon W. Bone, Bruce D. Hammock, Lyle K. Gaston, Susan K. Reed, H. H. Shorey

Entomology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Partial purification of a pheromone of the zooparasitic nematode Nippostrongylus brasiliensis was achieved. The polypeptide pheromone is degraded by Pronase while increased recovery is obtained after treatment with the protease inhibitors phenylmethylsulfonyl fluoride or l-1-tosylamide-2-phenylethyl chloromethyl ketone. The pheromone has a net negative charge, based on retention on DEAE cellulose and an approximate isoelectric point of 7.3. A combination of double treatments with phenylmethylsulfonyl fluoride and gel filtration enables storage up to nine days which yields biological activity similar to fresh, untreated chromatographic fractions.

Original language	English (US)
Pages (from-to)	297-308
Number of pages	12
Journal	Journal of Chemical Ecology
Volume	6
Issue number	2
DOIs	https://doi.org/10.1007/BF01402909
State	Published - Mar 1980

Keywords

Nematoda
nematode sex pheromones
pheromone characterization
pheromone purification

ASJC Scopus subject areas

Ecology
Biochemistry
Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01402909

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abstract = "Partial purification of a pheromone of the zooparasitic nematode Nippostrongylus brasiliensis was achieved. The polypeptide pheromone is degraded by Pronase while increased recovery is obtained after treatment with the protease inhibitors phenylmethylsulfonyl fluoride or l-1-tosylamide-2-phenylethyl chloromethyl ketone. The pheromone has a net negative charge, based on retention on DEAE cellulose and an approximate isoelectric point of 7.3. A combination of double treatments with phenylmethylsulfonyl fluoride and gel filtration enables storage up to nine days which yields biological activity similar to fresh, untreated chromatographic fractions.",

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AU - Bone, Leon W.

AU - Hammock, Bruce D.

AU - Gaston, Lyle K.

AU - Reed, Susan K.

AU - Shorey, H. H.

PY - 1980/3

Y1 - 1980/3

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AB - Partial purification of a pheromone of the zooparasitic nematode Nippostrongylus brasiliensis was achieved. The polypeptide pheromone is degraded by Pronase while increased recovery is obtained after treatment with the protease inhibitors phenylmethylsulfonyl fluoride or l-1-tosylamide-2-phenylethyl chloromethyl ketone. The pheromone has a net negative charge, based on retention on DEAE cellulose and an approximate isoelectric point of 7.3. A combination of double treatments with phenylmethylsulfonyl fluoride and gel filtration enables storage up to nine days which yields biological activity similar to fresh, untreated chromatographic fractions.

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