Partial purification of a pheromone of the zooparasitic nematode Nippostrongylus brasiliensis was achieved. The polypeptide pheromone is degraded by Pronase while increased recovery is obtained after treatment with the protease inhibitors phenylmethylsulfonyl fluoride or l-1-tosylamide-2-phenylethyl chloromethyl ketone. The pheromone has a net negative charge, based on retention on DEAE cellulose and an approximate isoelectric point of 7.3. A combination of double treatments with phenylmethylsulfonyl fluoride and gel filtration enables storage up to nine days which yields biological activity similar to fresh, untreated chromatographic fractions.
- nematode sex pheromones
- pheromone characterization
- pheromone purification
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)