Partial purification of a novel mitogen for oligodendroglia

B. Q. Kreider, J. B. Grinspan, M. B. Waterstone, G. T. Bramblett, B. Ances, M. Williams, J. Stern, V. M Y Lee, David E Pleasure

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A protein with a MW(app) of 50-70 kDa isolated from the salt extract of crude membranes from neonatal rat brain increases the numbers of oligodendroglia in mixed glial cultures prepared from neonatal rat cerebral white matter. After partial purification by ion exchange and gel exclusion chromatography, and elution from an SDS-polyacrylamide gel, this protein ('oligodendroglial trophic factor,' OTF) elicited half-maximal oligodendroglial recruitment at a concentration of 5 ng/mL. OTF is a mitogen for oligodendroglia, and to a lesser extent, for oligodendroglial progenitor (O2A) cells, but does not stimulate proliferation of astroglia, Schwann cells, or endoneurial fibroblasts. OTF, unlike platelet-derived growth factor (PDGF), is not an oligodendroglial survival factor. Antibodies against PDGF and basic fibroblast growth factor (bFGF) do not interfere with the accumulation of oligodendroglia induced by OTF. When OTF is given simultaneously with either PDGF or bFGF, there is an additive increase in the numbers of cells of the oligodendroglial lineage.

Original languageEnglish (US)
Pages (from-to)44-53
Number of pages10
JournalJournal of Neuroscience Research
Volume40
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • growth factor
  • lineage
  • mitogen
  • oligodendroglia
  • oligodendroglial trophic factor

ASJC Scopus subject areas

  • Neuroscience(all)

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  • Cite this

    Kreider, B. Q., Grinspan, J. B., Waterstone, M. B., Bramblett, G. T., Ances, B., Williams, M., Stern, J., Lee, V. M. Y., & Pleasure, D. E. (1995). Partial purification of a novel mitogen for oligodendroglia. Journal of Neuroscience Research, 40(1), 44-53. https://doi.org/10.1002/jnr.490400106