Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice

T. P K Nguyen, T. T. Lam, Stephen W Barthold, S. R. Telford, R. A. Flavell, E. Fikrig

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

We determined whether Borrelia burgdorferi outer surface proteins (Osps) E and F could elicit immune responses useful for a Lyme disease vaccine. Thirty days after challenge with B. burgdorferi, mice produced antibodies to OspE but not OspF, whereas antibodies to OspF were present in sera of mice obtained 90 days after infection. Examination of sera from patients with Lyme disease revealed antibodies to OspF in a small number (14%) of early-stage disease patients but in a majority (58%) of patients with late-stage disease, while antibodies to OspE were rarely detected in patients. Mice immunized with recombinant OspE or OspF produced high titers of antibodies to OspE or OspF, respectively. OspF-immunized mice were partially protected from both intradermal syringe challenge and tick-mediated transmission of B. burgdorferi while vaccination with OspE did not confer immunity. B. burgdorferi organisms were, however, substantially destroyed within ticks that engorged on either OspE- (75% reduction in the number of spirochetes within the ticks, compared with controls) or OspF (90% reduction in the number of spirochetes within the ticks)-immunized mice.

Original languageEnglish (US)
Pages (from-to)2079-2084
Number of pages6
JournalInfection and Immunity
Volume62
Issue number5
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Partial destruction of Borrelia burgdorferi within ticks that engorged on OspE- or OspF-immunized mice'. Together they form a unique fingerprint.

Cite this