Abstract
Parthenolide, the principal bio-active component of the herb feverfew (Tanacetum parthenium), has shown antileukemic activity. We evaluated the cell cycle status and the phosphorylation/activation of proteins involved in signal transduction in t(4;11) and non-t(4;11) acute lymphoblastic leukemia (ALL) cell lines after treatment with parthenolide. The cells were treated with the vehicle or 10 μM parthenolide for 2, 4, 6 and 8 h. As shown by flow cytometric analysis, parthenolide induced growth arrest at the S to G2/M phase transition. Using multiplex technology and Western blotting, we showed that the treatment with parthenolide within 0 to 10 h induced the phosphorylation of stress signaling proteins, including the p38 mitogen-activated protein kinase, the c-Jun N-terminal kinase, c-Jun, the heat shock protein 27 and protein kinase B. These data show that parthenolide induces a stress response leading to cell death and provide further evidence suggesting that parthenolide could be useful as a novel therapeutic agent against high risk ALL with chromosomal translocation t(4;11).
Original language | English (US) |
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Pages (from-to) | 1307-1313 |
Number of pages | 7 |
Journal | International Journal of Oncology |
Volume | 37 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2010 |
Keywords
- Acute lymphoblastic leukemia
- Parthenolide
- Signal transduction
- Stress response
ASJC Scopus subject areas
- Cancer Research
- Oncology