Parthenolide induces significant apoptosis and production of reactive oxygen species in high-risk pre-B leukemia cells

Susan J. Zunino, Jonathan M Ducore, David H. Storms

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We investigated whether parthenolide, the principal bioactive component of the herb feverfew (Tanacetum parthenium) induced apoptosis in pre-B acute lymphoblastic leukemia (ALL) lines, including cells carrying the t(4;11)(q21;q23) chromosomal translocation. Parthenolide induced rapid apoptotic cell death distinguished by loss of nuclear DNA, externalization of cell membrane phosphatidylserine, and depolarization of mitochondrial membranes at concentrations ranging from 5 to 100 μM. Using reactive oxygen species (ROS)-specific dyes, an increase in nitric oxide and superoxide anion was detected in the cells by 4 h after exposure to parthenolide. Parthenolide-induced elevation of hypochlorite anion was observed only in the two t(4;11) lines. These data suggest parthenolide may have potential as a potent and novel therapeutic agent against pre-B ALLs.

Original languageEnglish (US)
Pages (from-to)119-127
Number of pages9
JournalCancer Letters
Volume254
Issue number1
DOIs
StatePublished - Aug 28 2007

Fingerprint

Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
Reactive Oxygen Species
Apoptosis
Tanacetum parthenium
Hypochlorous Acid
Genetic Translocation
Phosphatidylserines
Mitochondrial Membranes
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Superoxides
Anions
Nitric Oxide
Cell Death
Coloring Agents
Cell Membrane
parthenolide
DNA

Keywords

  • Apoptosis
  • Leukemia
  • Mitochondria
  • Parthenolide
  • Reactive oxygen species
  • ROS

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Parthenolide induces significant apoptosis and production of reactive oxygen species in high-risk pre-B leukemia cells. / Zunino, Susan J.; Ducore, Jonathan M; Storms, David H.

In: Cancer Letters, Vol. 254, No. 1, 28.08.2007, p. 119-127.

Research output: Contribution to journalArticle

@article{2fc833c2aded41de869a313d9686e523,
title = "Parthenolide induces significant apoptosis and production of reactive oxygen species in high-risk pre-B leukemia cells",
abstract = "We investigated whether parthenolide, the principal bioactive component of the herb feverfew (Tanacetum parthenium) induced apoptosis in pre-B acute lymphoblastic leukemia (ALL) lines, including cells carrying the t(4;11)(q21;q23) chromosomal translocation. Parthenolide induced rapid apoptotic cell death distinguished by loss of nuclear DNA, externalization of cell membrane phosphatidylserine, and depolarization of mitochondrial membranes at concentrations ranging from 5 to 100 μM. Using reactive oxygen species (ROS)-specific dyes, an increase in nitric oxide and superoxide anion was detected in the cells by 4 h after exposure to parthenolide. Parthenolide-induced elevation of hypochlorite anion was observed only in the two t(4;11) lines. These data suggest parthenolide may have potential as a potent and novel therapeutic agent against pre-B ALLs.",
keywords = "Apoptosis, Leukemia, Mitochondria, Parthenolide, Reactive oxygen species, ROS",
author = "Zunino, {Susan J.} and Ducore, {Jonathan M} and Storms, {David H.}",
year = "2007",
month = "8",
day = "28",
doi = "10.1016/j.canlet.2007.03.002",
language = "English (US)",
volume = "254",
pages = "119--127",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Parthenolide induces significant apoptosis and production of reactive oxygen species in high-risk pre-B leukemia cells

AU - Zunino, Susan J.

AU - Ducore, Jonathan M

AU - Storms, David H.

PY - 2007/8/28

Y1 - 2007/8/28

N2 - We investigated whether parthenolide, the principal bioactive component of the herb feverfew (Tanacetum parthenium) induced apoptosis in pre-B acute lymphoblastic leukemia (ALL) lines, including cells carrying the t(4;11)(q21;q23) chromosomal translocation. Parthenolide induced rapid apoptotic cell death distinguished by loss of nuclear DNA, externalization of cell membrane phosphatidylserine, and depolarization of mitochondrial membranes at concentrations ranging from 5 to 100 μM. Using reactive oxygen species (ROS)-specific dyes, an increase in nitric oxide and superoxide anion was detected in the cells by 4 h after exposure to parthenolide. Parthenolide-induced elevation of hypochlorite anion was observed only in the two t(4;11) lines. These data suggest parthenolide may have potential as a potent and novel therapeutic agent against pre-B ALLs.

AB - We investigated whether parthenolide, the principal bioactive component of the herb feverfew (Tanacetum parthenium) induced apoptosis in pre-B acute lymphoblastic leukemia (ALL) lines, including cells carrying the t(4;11)(q21;q23) chromosomal translocation. Parthenolide induced rapid apoptotic cell death distinguished by loss of nuclear DNA, externalization of cell membrane phosphatidylserine, and depolarization of mitochondrial membranes at concentrations ranging from 5 to 100 μM. Using reactive oxygen species (ROS)-specific dyes, an increase in nitric oxide and superoxide anion was detected in the cells by 4 h after exposure to parthenolide. Parthenolide-induced elevation of hypochlorite anion was observed only in the two t(4;11) lines. These data suggest parthenolide may have potential as a potent and novel therapeutic agent against pre-B ALLs.

KW - Apoptosis

KW - Leukemia

KW - Mitochondria

KW - Parthenolide

KW - Reactive oxygen species

KW - ROS

UR - http://www.scopus.com/inward/record.url?scp=34447319405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447319405&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2007.03.002

DO - 10.1016/j.canlet.2007.03.002

M3 - Article

C2 - 17470383

AN - SCOPUS:34447319405

VL - 254

SP - 119

EP - 127

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -