Parthenolide attenuates LPS-induced activation of NF-κB in a time-dependent manner in rat myocardium

Hong Xie, Chen Wang, Xuemei Wu, Xia Liu, Shigang Qiao, Chunfeng Liu, Hong Liu

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8 Scopus citations

Abstract

Parthenolide (PTN), a selective nuclear factor kappa B (NF-κB) inhibitor, has been used extensively to inhibit NF-κB activation. The duration of the inhibitory effect of PTN on NF-κB in vivo remains unclear. This study was to determine whether a lipopolysaccharide (LPS) challenge 6, 12 and 24 h after the administration of PTN could activate NF-κB. Rats were devided into five groups. The rats in the PTN, PTN+LPS and DMSO groups were injected intraperitoneally with PTN or DMSO. After 6, 12 or 24 h, LPS was administered in LPS and PTN+LPS groups. The expressions of NF-κB p50, IκBα and p-IκBα were inhibited in both PTN and PTN+LPS group at end of 6 and 12 h and no effects at 24 h. In summary, myocardial NF-κB expression occurs 1 h after the administration of LPS. PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo.

Original languageEnglish (US)
Pages (from-to)37-43
Number of pages7
JournalJournal of Biomedical Research
Volume26
Issue number1
DOIs
StatePublished - Jan 2012

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Keywords

  • Lipopolysaccharide
  • Myocardium
  • Nuclear-factor-κB
  • Parthenolide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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