PARP-1 deficiency increases the severity of disease in a mouse model of multiple sclerosis

Vimal Selvaraj, Mangala M. Soundapandian, Olga Chechneva, Ambrose J. Williams, Maxim K. Sidorov, Athena Soulika, David E Pleasure, Wenbin Deng

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) has been implicated in the pathogenesis of several central nervous system (CNS) disorders. However, the role of PARP-1 in autoimmune CNS injury remains poorly understood. Therefore, we studied experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis in mice with a targeted deletion of PARP-1. We identified inherent physiological abnormalities in the circulating and splenic immune composition between PARP-1-/- and wild type (WT) mice. Upon EAE induction, PARP-1-/- mice had an earlier onset and developed a more severe EAE compared with WT cohorts. Splenic response was significantly higher in PARP-1-/- mice largely because of B cell expansion. Although formation of Th1 and Th17 effector T lymphocytes was unaffected, PARP-1-/- mice had significantly earlier CD4+ T lymphocyte and macrophage infiltration into the CNS during EAE. However, we did not detect significant differences in cytokine profiles between PARP-1-/- and WT spinal cords at the peak of EAE. Expression analysis of different PARP isozymes in EAE spinal cords showed that PARP-1 was down-regulated in WT mice and that PARP-3 but not PARP-2 was dramatically up-regulated in both PARP-1-/- and WT mice, suggesting that these PARP isozymes could have distinct roles in different CNS pathologies. Together, our results indicate that PARP-1 plays an important role in regulating the physiological immune composition and in immune modulation during EAE; our finding identifies a new aspect of immune regulation by PARPs in autoimmune CNS pathology.

Original languageEnglish (US)
Pages (from-to)26070-26084
Number of pages15
JournalJournal of Biological Chemistry
Volume284
Issue number38
DOIs
StatePublished - Sep 18 2009

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Autoimmune Experimental Encephalomyelitis
Poly(ADP-ribose) Polymerases
Multiple Sclerosis
Neurology
Central Nervous System
T-cells
Pathology
Isoenzymes
Spinal Cord
Nervous System Trauma
T-Lymphocytes
Central Nervous System Diseases
Poly (ADP-Ribose) Polymerase-1
Macrophages
Chemical analysis
Infiltration
B-Lymphocytes
Mouse Parp1 protein
Cytokines
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

PARP-1 deficiency increases the severity of disease in a mouse model of multiple sclerosis. / Selvaraj, Vimal; Soundapandian, Mangala M.; Chechneva, Olga; Williams, Ambrose J.; Sidorov, Maxim K.; Soulika, Athena; Pleasure, David E; Deng, Wenbin.

In: Journal of Biological Chemistry, Vol. 284, No. 38, 18.09.2009, p. 26070-26084.

Research output: Contribution to journalArticle

Selvaraj, Vimal ; Soundapandian, Mangala M. ; Chechneva, Olga ; Williams, Ambrose J. ; Sidorov, Maxim K. ; Soulika, Athena ; Pleasure, David E ; Deng, Wenbin. / PARP-1 deficiency increases the severity of disease in a mouse model of multiple sclerosis. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 38. pp. 26070-26084.
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